中药防哮饮早期预治疗对哮喘小鼠血清IL-10水平影响的研究.pdfVIP

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中药防哮饮早期预治疗对哮喘小鼠血清IL-10水平影响的研究.pdf

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中药防哮饮早期预治疗对哮喘小鼠血清IL-10水平影响的研究

目录中文摘要 ··············································1 英文摘要 ··············································3 引言 ···············································6 正文 ···············································7 1.实验材料 ············································7 1.1 实验对象 ··········································7 1.2主要试剂与药品 ····································7 1.3 主要仪器和设备 ··································7 1.4 药品配制 ·········································8 2.实验方法 ············································8 2.1 实验分组及给药 ···································8 2.2 哮喘模型的制备方法 ······························8 2.3 观察指标 ··········································9 2.4 标本处理 ··········································9 3.统计方法 ··········································· 12 4.实验结果 ··········································· 12 4.1 小鼠行为学观察 ···································12 4.2 各组小鼠外周血血清 IL-10 含量的比较 ·············13 4.3 各组小鼠BALF 中EOS 百分比的比较 ·················14 5.讨论 ················································15 5.1 哮喘的发病机理 ···································16 5.2 中医对哮喘的认识 ·································19 6.哮喘模型的评价 ·····································28 6.1 实验动物的选择 ···································29 6.2 变应的选择 ·······································29 6.3 哮喘模型的做作方法 ······························29 7.存在的的问题及展望 ································30 结论 ··············································32 参考文献 ··············································33 附录 ···············································38 综述 ···············································41 致谢 ···············································57 攻读学位期间发表的学术论文目录 ·····················58 作者声明 ··············································59 个人简历 ··············································60 中文摘要目的: 本研究旨在通过动物实验观察中药防哮饮早期干预治疗对哮喘小鼠血清白细胞介素-10(IL-10)、支气管肺泡