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寒痹康汤对实验关节炎大鼠血清TNF-α及NO的影响
目 录
中文摘要 ················································1
英文摘要 ················································3
引 言 ················································5
正 文 ················································7
1.实验材料与方法 ······································7
1.1 实验动物·············································7
1.2 动物室 ··············································7
1.3 药物与试剂 ···········································7
1.4 主要仪器 ·············································7
1.5 实验方法 ············································7
1.6 指标的检测 ········································9
1.7 数据统计 ··········································11
2.结果和分析 ··········································11
2.1 动物观察 ···········································11
2.2关节炎指数评定结果 ·····························12
2.3 大鼠足趾肿胀度的分析 ····························12
2.4 TNF- α的水平分析····································13
2.5 NO 水平分析 ···································13
2.6 结果分析 ·········································13
3.讨论·················································14
4.存在的问题与展望 ·································36
结 论 ···············································37
参考文献 ··············································38
综 述 ···············································44
致 谢 ···············································63
攻读学位期间发表的学术论文题目 ····················64
作者声明 ··············································65
个人简历 ··············································66
中 文 摘 要
目 的：
通过本课题的研究观察寒痹康汤对实验性关节炎大鼠血清肿瘤坏死
因子- α(TNF- α)及一氧化氮水平（NO ）的影响，初步探讨寒痹康汤治疗
类风湿关节炎（RA ）的作用机制,并为临床上运用中医药防治类风湿关节
炎提供实验依据。
方 法：
将 36 只SD 大鼠随机分为4 组(寒痹康汤组、甲氨喋呤组、模型组、
正常对照组)。除正常对照组外，模型组、寒痹康汤组和甲氨喋呤组皮下
注射牛Ⅱ型胶原蛋白加弗氏不完全佐剂诱导实验性关节炎模型，造模前
测量所有大鼠足趾部厚度（mm ）。于造模的第8 天先测量大鼠足趾部厚度，
寒痹康汤组和甲氨喋呤组分别灌服寒痹康汤、甲氨喋呤，寒痹康汤组每
天 2 次，甲氨喋呤组每周一次；模型组每天灌服与寒痹康汤组等量的蒸
馏水；正常组自由饮水。连续给药至第
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