不同类型缺氧Different Types of Hypoxia(英文版).docVIP

Lab Report of Medical Functional Experimentation Date： Title Different Types of Hypoxia Score Experimenters Grade and specialized field Preventive Medicine，Group 10 1. Purpose A. To understand the classification principle of hypoxia by duplication of animal model of different types of hypoxia B. To understand the manifestation character of different types of hypoxia through the observation of the variation of respiratory rhythm and skin and mucosa color of animal with hypoxia. 2. Animal and Materials (1)Animal: Adult mice, weighing about 20gram, male or female (2)Apparatus: Ginsberg Scientific Reagent Bottles - Flint - Wide Mouth - Ground Glass Stopper, carbon monoxide generating device, alcohol lamp)、syringe, needles, scissors, tissue forceps. (3)Drugs and reagents: Soda lime, formic acid solution, concentrated sulfuric acid solution, 5% sodium nitrite solution, 1% methylene blue solution. 3. Methods and Procedures （1）Observe the normal manifestation of the mouse placed in a wide-necked bottle, which is attached to a set of carbon monoxide generation device prepared beforehand. Take 3 ml of formic acid solution into a test tube by calibration pipette, followed by slow addition of 2 ml of concentrated sulfuric acid solution along the inclined tube wall. Seal the tube with rubber stopper and observe the same indexes as above. It should be noticed that overheating the test tube by alcohol burner is dangerous due to possible liquid seething f speeding up generation of carbon monoxide is necessary. (Furthermore, carbon monoxide generated too rapid and too much would cause the mouse die rapidly without evident change of blood color.) 2) Hypoxia of sodium nitrite poisoning: Take 2 mice with same body weight and observe their normal indexes. Give each mouse 0.3 ml of 5% sodium nitrite solution by intraperitoneal injection respectively, immediately followed by intraperitoneal injection o