细胞分子生物学9分子遗传学技术与基因组学课件.pptVIP

9 MOLECULAR GENETICTECHNIQUES AND GENOMICS Overview of two strategies for determining the function, location, and primary structure of proteins DNA Cloning by Recombinant DNA Methods The polymerase chain reaction (PCR) is widely used to amplify DNA regions of known sequences Some eukaryotic proteins can be produced in E. coli cells from plasmid vectors containing the lac promoter lacZ:β-半乳糖苷酶基因 granulocyte colony-stimulating factor (G-CSF):粒细胞集落刺激因子 DNA Sequencing Stored Sequences Suggest Functions of Newly Identified Genes and Proteins Comparison of the regions of human NF1 protein and S. c