生物信息学实习1宋捷.docVIP

生物信息学实习一 宋捷 生技 122 2012014069 作业： 1. Do the following search with Entrez at NCBI , describe the steps and answer the questions. The number of nucleotide sequence records associated with breast cancer 在entre中搜索乳腺癌加引号，下滑找到核酸相关 Number of the above sequence records that are from the RefSeq database 在结果左面的菜单里显示相关数据库该词条相关数量 3) Number of nucleotide sequences associated with breast cancer that are mRNAs mRNA(20,134) 4) Number of the above mRNA sequence records that are in RefSeq and the words breast cancer appears in their titles 语法： breast cancer[All Fields] AND (biomol_mrna[PROP] AND refseq[filter]) 5) Number of Human RefSeq mRNA sequence records with the words breast cancer in their titles 语法： (human[Organism]) AND (breast cancer[All Fields] AND biomol_mrna[PROP] AND refseq[filter]) Results: 1 to 20 of 3603 6) Number of Human gene BRCA1 RefSeq mRNA sequence records with the words breast cancer in their titles 语法： (((breast cancer[All Fields] AND biomol_mrna[PROP] AND refseq[filter])) AND brca1[Gene Name]) AND human[Organism] Results: 5 7) Accession number of the mRNA record for human BRCA1, transcript variant 1, in RefSeq. Accession: NM_007294.3 GI:237757283 8) Total number of nucleotides reported in this record 7,224 bp linear mRNA 9) Exact chromosomal location of BRCA1, transcript variant 1 on the human genome /map=17q21 10) Number of times this sequence was updated 版本号从.1~.3 应该是共有三次录入 11) Total number of amino acids that this mRNA encodes 数的….1863（word不含空格） 点到ID号里 有该蛋白质序列的详细信息 12) Identify the last amino acid in the encoded protein Y 是酪氨酸 13) Total number of Exons and Introns in this gene variant (BRCA1, transcript variant 1) Exon 23个 misc_feature 26个 网页搜索数 14) Total number of exons (partial or complete) in the 5’UTR of BRCA1, transcript variant 1 5’非编码区，在CDS前有2个外显子 15) Total number of exons (partial or complete) in the 3’UTR of BRCA1, transcript variant 1 CDS后，在其后有一个外显子 16) BRCA1, transcript variant 1 encode