荧光定量PCR原理应用及StratageneMxP介绍资料.ppt

* * * * * * * * * * * * * * * * 多种应用模式 软件的结构 PCR板的设定 PCR程序设定 运行时的状态 实时扩增数据 软件自行优化的数据分析 Adaptive baseline correction Amplification-based threshold 标 准 曲 线 相对定量Results Display 4 fold down 3 fold up 基因分型结果显示 User defined genotypes, color coded populations, and automatic calling on text report “只读板”实验结果 结果文本显示 Text Report or raw data can be exported to Excel or a text file Customize report 多种格式输出结果 Text Report Excel spreadsheet PowerPoint presentation Any screen shot Brilliant? QRT-PCR and QPCR Reagents Master mixes and reagent kits for any fluorescent chemistry Compatible with sequence-specific probes SYBR Green kits DNA Amplification Brilliant? SYBR? Green QPCR Master Mix Brilliant? SYBR? Green QPCR Core Reagent Kit RNA Amplification Brilliant? SYBR? Green QPCR Master Mix, 1-Step Brilliant? SYBR? Green QPCR Reagents DNA Amplification Brilliant? QPCR Core Reagent Kit Brilliant? Plus Core Reagent Kit Brilliant? QPCR Master Mix 2-step RT-PCR RNA Amplification Brilliant? QRT-PCR Core Reagent Kit, 1 - Step Brilliant? QRT-PCR Master Mix Kit, 1 - Step Brilliant? QRT-PCR Plus Core Reagent Kit, 1 - Step 1-step RT-PCR Brilliant? QRT-PCR Core Reagent Kit, 2 - Step Brilliant? QRT-PCR Plus Core Reagent Kit, 2 - Step Brilliant? Probe-based QPCR Reagents 2-fold discrimination using the Brilliant SYBR QPCR master mix on the Mx3000P instrument Ct variation 0.5Cts 10,000 copies 5,000 copies Brilliant? QPCR Kits Target = Beta actin / Template = Human genomic DNA 36 wells of 10,000 copies with a Ct variation of 0.5 Cts and 36 wells of 5,000 copies with Ct variation of 0.5Cts 引物探针设计软件 设计SYBR Green 引物, TaqMan? 探针, FRET 探针和 Molecular beacons（分子信标） 快速,简单,功能强大 独特优点 能高效设计双标记探针(Taqman,FRET探针,分子信标等) 优化的引物设计能适应不同的荧光定量PCR方法 能进行等位基因分析与多重定量PCR探针设计 有二级结构分析功能,能图形化显示各种位置与结构 内置BLAST连接 BeaconDesigner 4.0 工作界面 Fast Track QPCR 培训项目 优势： 快速有效的 QPCR 培训； 更高层次的QPCR应用培训； 能满足客户的不同要求； 从入门到高级的完全覆盖； 用户能直接咨询Stratagene的QPCR应用 专家 /fasttrack Fast Track培训内容 Web-seminar（网络