大白菜—结球甘蓝单体异附加系AC4及其后代小孢子植株的合成与鉴定课件分析.docVIP

大白菜—结球甘蓝单体异附加系AC4及其后代小孢子的鉴定 ( （河北农业大学园艺学院，河北保定 071000） 摘 要：大白菜—结球甘蓝AAC，2n=29）与二倍体大白菜85-1（AA，2n=20）回交一代为材料，经过染色体核型分析，从中筛选出大白菜—结球甘蓝AC4的亲本大白菜85-1和结球甘蓝11-1为试材，位于结球甘蓝C基因组9个连锁群的902个InDel标记进行筛选，得到了结球甘蓝相对于大白菜多态性引物6个多态性引物进一步对AC4后代小孢子进行鉴定，AC4外源蓝连锁群C05C08、C02、C03C04；157份小孢子为精确鉴定添加甘蓝染色体片段的大白菜易位系奠定了基础。大白菜；结球甘蓝；单体异附加系；InDel标记；小孢子Creation and Identification of Chinese cabbage-cabbage Monosomic Alien Addition Line AC4 and its progeny plants derived from microspore culture DONG Hui，GU Ai-xia， WANG Yan-hua，SHEN Shu-xing，and ZHAO Jian-jun* （College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071000，China） Abstract: A monosomic addition line AC4 (Chinese cabbage with chromosome No. 4 from cabbage) derived from the 1st backcrossing generation between Chinese cabbage-cabbage allotriploid (AAC, 2n=29) and diploid Chinese cabbage (AA，2n=20) was obtained by cytology identification. DNAs from AC4 and its parents Chinese cabbage 85-1 and cabbage 11-1 were used for PCR screening, 674 polymorphic primers between 85-1 and 11-1 were identified from 902 Indel markers. The AC4 progeny plants derived from microspors were further identified by polymorphic primers. The results showed that exogenous genetic component mainly contained a large part of chromosome C05 and a small part of chromosome C08、C02、C03C04. 1-12 Cabbage genomic segments were found from 157 plants derived from microspores, in which 9 plants had only one Cabbage genomic segment with percentage of 5.73%. This research would be useful for identifying precisely Chinese cabbage translocation lines introduced cabbage chromosome fragments, and for studying expression patterns of the exogenous Cabbage genes under Chinese cabbage genetic background. Key words: Chinese cabbage; Cabbage; InDel markers; Monosomic addition line; Microspore 异附加系（alien addition lines）是指通过人工远缘杂交，然后自交或回交，使作物的染色体组附加了异种或异属的1条或几条染色体而形成的植物新系统。根据附加到宿主基因组的染色体不同可分为单体异附加系、双体异附加系、多重异附加系或端体附加系。目前已经创建了多种作物（如小麦、燕麦、水稻、葱、马铃薯、黄瓜、甜菜等）的异附