生化工程,第三章固定化酶反应动力学课件.pptVIP

Immobilized Enzyme Systems Definition §3.4 Immobilized Enzyme Kinetics 例3-1某酶固定在无微孔球形基质上，其本征动力学参数为Vmax=4X10-5mol/(L S),Km=2X10-5mol/L. [Sb]= 1X10-5 mol/L, kLa= 0.4 s-1, 求[SS]和底物在固定化酶外表面上的反应速率？ (I). Concentration distribution in A Porous Matrix (II). Thiele modulus For a spherical porous matrix, first-order reaction rate, Definition of Thiele modulus It’s a measure of the extent of internal diffusion resistances Thiele modulus calculation When internal diffusion limits the enzymatic reaction rate, the rate constant and values are not true intrinsic rate constants, but apparent o