花生中植物螯合肽PC的研究本科毕业论文讲义.docVIP

题 目： 花生中植物螯合肽PC的研究 学 院： 专 业： 姓 名： 指导教师： 完成日期： 摘要 重金属是我们熟知的环境污染物之一，， ，，镉被人体吸收后，在体内镉硫蛋白，镉硫蛋白选择性于肾、肝中。其中，进入体内1/3的镉肾脏吸收，是镉中毒的靶器官。其它脏器如胰、脾、甲状腺和毛发等也一定量。镉在体内可与含氨基、羟基、硫基的蛋白质分子结合，抑制许多酶系统，从而肝、肾器官中酶系统的正常功能。由于镉损伤肾小管，病出现糖尿、蛋白尿和氨基酸尿。使骨骼的代谢受阻，造成骨质疏松、萎缩等一系列症状。1.5%的三氟乙酸检测了花生中在不同浓度镉诱导下形成的植物螯合肽含量。在以上的实验条件下，检出限为0.25mg/L；0.60-0.25mg/L( R2=0.9988)之间，回收率在80% 以上，灵敏度相对较高。选用该方式对花生中的螯合肽做定量分析，证明了花生中有植物螯合肽。结果表明，重金属镉污染的花生中都检出不同浓度的植物螯合肽。 本实验的目的在于为重金属镉污染的减弱做一份贡献。 关键字：镉污染；花生；植物螯合肽（PC）；高效液相色谱-质谱 Abstract heavy metals is known as?one of environmental pollutants, in recent years, the development of industrials, the use of pesticides, fertilizers, makes the heavy metals in the aquatic and soil pollution becomes more and more serious, and heavy metals are accumulated in the plant organs, the finally, human is persecuted by heavy metals through the food chain. Using plants to reduce heavy metal pollution is a hotspot of research and development at home and abroad. The study found that cadmium absorbed by the body will turn into cadmium sulfide protein, cadmium sulfide is a selective protein, can be accumulated in kidney, a third of the cadmium can be absorbed by the kidney, the kidney is the target organ of cadmium poisoning. Other organs such as pancreas, spleen, thyroid gland and hair also be accumulated a certain amount. Cadmium in the body will be united by protein molecules containing amino, hydroxyl, inhibition of many enzyme system, so that the normal function of the liver and kidney organs in enzyme system will be damaged. Due to the cadmium, the patient can appear the symptom of diabetes, protein and amino acid urine, More seriously, which can make bone metabolism, cause osteoporosis, contraction and a series of symptoms, the harm of cadmium has been greatly concerned. In th