实验二、触发器及应用课程.docVIP

《数字电路》 实验指导书 实验报告参考规范 实验题目: 班级________姓名________学号_________日期_______ 指导教师:_____________ 实验目的 实验内容 实验步骤 四?实验结果分析 五?实验小结 实验二?触发器及应用 实验目的和要求 掌握基本触发器的功能和应用 实验内容 (1)?根据D触发器和JK触发器的特性表逐项测定触发器的逻辑功能? (2)?用JK触发器设计一个二分频电路? 3.实验提示 (1)主要器件为74LS74和74LS112? (2)利用JK触发器设计成二分频电路即是将JK触发器设计成T触发器,比较二者的特性方程可得当 J=K时JK触发器即转换为T触发器? 4. 实验报告要求: 实验目的与要求? 实验中所使用的器件引脚图?功能表? 实验中实验电路的设计方法与逻辑图? 绘制利用74LS74 和74LS112实现二分频电路的工作波形? 实验体会与对实验内容的改进要求及设想方案? Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH, USA). For RAH-specific T cell staining, spleens were harvested seven days after the immunization