利用SSR标记分析27个玉米群体遗传关系_段运平.pdfVIP

2006,39(6):1102-1113 Scientia Agricultura Sinica 1 100081 2 3 030031 038000) The Genetic Diversity Among 27 Maize Populations Based on SSR Data 2 ,3 3 1 1 1 1 DUAN Yun-ping , CHEN Wei-guo , LI Ming-shun , LI Xin-hai , LIU Xue , TIAN Qing-zhen , 1 1 BAI Li , ZHANG Shi-huang 1 ( Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081 ; 2Institute of Crop Genetics, Shanxi Academy of Agricultural Sciences, Taiyuan 030031; 3Shanxi Agricultural University, Taigu 038000) Abstract: Objective In this study, the genetic diversity and relationship among 27 maize (Zea mays L. ) populations were analyzed based on the data of SSRs. MethodFour bulk DNA samples for each population were extracted from leaf mixture of 10 individuals for each sample. The genetic diversity of 27 maize populations, including 13 domestic and 14 CIMMYT populations were evaluated by 71 SSR primers, which are averagely distributed on 10 chromosomes of maize. ResultOn the 71 SSR loci, a total of 389 alleles were detected in these populations. At each locus, 2 to 19 alleles could be detected, with an average of 5.48. Mean polymorphism information content was 0.66. The number of polymorphic alleles differed in domestic and CIMMY populations, which were 329 for domestic