基于纳米金和硫堇固定酶的过氧化氢生物传感器答辩人陈课件摘要.ppt

基于纳米金和硫堇固定酶 的过氧化氢生物传感器 答辩人：陈贤光（03应化） 指导老师：童叶翔 教授 邹小勇 教授2006.6 广州 Hydrogen Peroxide Biosensor Based on Immobilizing Enzyme by Gold Nanoparticles and Thionine① CHEN, Xian-Guang ZOU, Xiao-Yong TONG, Ye-Xiang*School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China 1. Introduction1.1 Mechanism of Hydrogen Peroxide Biosensor 1. Introduction 1.2 Fabrication of hydrogen peroxide biosensor made in our work 1. Introduction 1.3 Catalytic reaction of H2O2 appear in the system 2. Results and Discussion 2.1 Prepare GNs by sol-gel method 2. Results and Discussion 2.2 Electrochemical impedance spectrum (EIS) Fig. 2 Nyquist plot of EIS of the electrodes (a) and equivalent circuit (b) of the biosensor. 2. Results and Discussion 2.3 Atomic force microscopy (AFM) images 2. Results and Discussion 2.4 Cyclic voltammgrams (CVs) 2. Results and Discussion 2.5 Some factors effect on the response currents 2. Results and Discussion 2.5 Some factors effect on the response currents 2. Results and Discussion 2.6 Spectra study of the effect between HRP and GNs 2. Results and Discussion 2.6 Spectra study of the effect between HRP and GNs Fig. 11 Circular dichroism spectra of HRP in the absence (a) and presence (b) of GNs. Other conditions are as Fig. 10. 2. Results and Discussion 2.7 Applied performance of the biosensor to H2O2 2. Results and Discussion 2.8 Applied performance of the bienzymebased  biosensor to glucose 2. Results and Discussion 2.8 Applied performance of the bienzymebased  biosensor to glucose 3. Conclusion 4. Production 5. Acknowledgement * * ① This paper will submit to Acta Chim. Sinica . Guilbault G. G. , et. al. Anal. Chim. Acta. , 1973, 64: 439-445. Fig. 1 TEM image of GNs. ( d = 20 nm ) (a) (b) Rapp : electrode apparent resistance; Rsol : solution resistance; Rct : charge transfer resi