两种鲤鱼胰凝乳蛋白酶原基因的电子克隆及其分析.pdfVIP

生物技术通报 BIO TE CHN OLOG YB ULLETIN 2011 1 陈文波李卫国 (, 454000) : 为研究胰凝乳蛋白酶原在鱼类中的生理功能和作用机制,利用生 信息学的方法, 成功获得了鲤鱼两种胰凝 乳蛋白酶原的 cDNA序列 (ccCHTR1和 ccCHTR2)并对其进行序列分析结果显示, ccCHTR1 cDNA含有 792 bp的开放阅读 框, 编码 263 个氨基酸; ccCHTR2 cDNA含有 798 bp的开放阅读框, 编码 265 个氨基酸二者氨基端均含有 18 个氨基酸组成 的信号肽, 同时,在成熟肽的第 15和 16 个氨基酸(R-I)之间存在 一个切割位点氨基酸比对结果显示, ccCHTR1和 ccCHTR2 具备胰凝乳蛋白酶原的保守结构特征, 同时二者有 728%的同源性,且都与斑马鱼有最高的同源性,分别是 933%和 735% 进化分析显示, 二者分别与斑马鱼和鳕鱼亲缘关系最近, 与哺乳动 的亲缘关系较远 : 鲤鱼 胰凝乳蛋白酶原 电子克隆 表达序列标签 生 信息学 Cloning and Analysis of Two Common Carp Chymotrypsinogen Genes in silico Chen enbo Li eiguo (School of R esource andEnvironmen,tH enan Polytechnic University, Jiaozuo 454000) Abstrac:t To study the physiological funtions and mechanism of chymotrypsinogen in fish, we got two common carp chymot- rypsinogen cDNAs (ccCHTR1 and ccCHTR2) successfully using in silico cloning, and then analysed by themethod of bioinformatics. The results showed that ccCHTR1 cDNA contained an open reading frame(ORF) 792 bp in length, encoding 263 am ino acids, and ccCHTR2 cDNA had an ORF 798 bp long, encoding265 am ino acids. Both had a signalpeptide consisted of 18 am ino acids, and there wasa cleavagesitebetweenthe resudes 15 and16 in thematuredprotein. The alignmentandhomology analysisbased on theamino acid sequencesrevealed that ccCHTR1 and ccCHTR2 had conversed structural characteristics. It had the identityof72. 8% bewt een the two common carp chymotrypsinogen, and the highest homology of 933% and 735% to zebrafish. The phylogenetic tree showed the two chymotrypsinogensof common carpwere closely related to zebrafish andAtlantic cod, but less related to themammalian species. K ey w ords: Common carp