亚麻直接分化再生系统的初步建立.pdfVIP

生物技术通报 BI OTECHN OLOG Y B ULLETI N 2005 3 姬妍茹 田玉杰 宋淑敏 关向军 ( , 150 111) : 在亚麻愈伤分化再生系统基础上对诱导培养基 生根培养基加以改良创立了一套新的亚麻再生 系统 直接分化再生系统该系统使外植体不经过愈伤组织阶段直接获得分化芽, 且在 个别品种上绿苗诱导率 最高达242. 11% , 移栽成活率达60% 以上, 这为纤维亚麻遗传转化提供了良好的受体系统 : 亚麻 再生系统 直接分化 遗传转化 受体系统 Preliminary Creation on Regenerational Flax System of Shoot Differentiate Directly from Explant Ji Y anru T ian Y ujie Song Shumin Guan Xiangjun ( H ei longj i an g P rov ince r aw M at er ial an d I n dust ry I nsti t ute of f la x , S huangc heng 150 111) Abstract: A new reg ener ationa sy stem of f iber f ax w as created, it w as ca ed direct diff er entiation r egenera tiona system . T his sy st em base an impro vement o n reg ener ationa system of shoo t diff er entiated fro m ca u s. O n the medium of dir ect diff er ent iatio n, the ex p ant didn t produce ca u s, but had direct diff erentiation of sho ot , mor eover on so me genoty pe, the percent age of shoot inducement reach 242 . 11% , the per centag e of transp ant w as mor e than 60% . T his w i be pr ov ide a g oo d accepto r sy stem fo r g enetic t ransfo rm o n f iber f ax . Key w ords: F ax R eg eneratio na system Dir ect diff erent iatio n G enetic t ransfo rm A ccepto r system , [ 1] , , , , ; , , , , [ 2] , , , , 1 1. 1 植物材料及处理方法 ( ) A riane( A r) 75% 30 , 0 . 1% H gC 2 10 , 3~ 4 , B5 , 20~ 25 , 8 , 7 , ( ) , ( 3~ 5m m) , ( 1cm ) 1. 2 培养基 : : ( 197 1) , , , 42 生物技术通报Biotechnol ogy Bull et in 2005 3 5 6 1. 2. 1 B + N + IA A ( 4.