毛竹抗逆锌指蛋白基因cDNA克隆及序列分析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 1 cDNA 刘志伟 张智俊 杨丽 (, 311300) : 根据水稻抗逆相关锌指蛋白基因的保守区序列设计 引 , 以毛竹基因组 DNA和 cDNA为模板, 采用 PCR方 法, 成功扩增出 1 个含有完整阅读框架的 cDNA序列,长度为 495 bp,序列无内含子, 共编码 164 个氨基酸, 将其命名为 PeZFP 基因( GenBank登录号: FJ472953) 氨基酸序列(GenBank登录号: AC 01101)的分析结果表明, PeZFP与其他锌指结构蛋白有 较高的同源性, 同水稻锌指结构抗逆蛋白 OSIAP1序列相似性高达 877% , 且其序列 C端具有典型的 AN1类型的锌指结构 Cx24Cx912Cx2Cx4Cx2Hx5HxC,在 N端具有典型的 A20类型锌指蛋白结构, 推测此PeZFP为植 抗逆 OsIAP1类似基因,在功 能上与毛竹抗逆性相关 : 毛竹 锌指蛋白 克隆 cDNA Cloning and Sequence Analysis of a Zincfinger Protein Gene Involved in Stresstolerance inPhy llostachys edulis iu Zhwi ei Zhang Zhijun Yang i (The Key Laboratory f orM odern Silvicultural Technology of Zhej iang P rovince, Zhej iang F orestry Co llege, L inan 311300) Abstrac:t A zincfinger protein gene from hy llos tachys edulis, nam ed as PeZFP( GenBank accession number: FJ472953), w as cloned through PCR using prmi ers designed according to the zincfinger protein genes conserved region of other p lantThe coding region of the genom ic clone of PeZFP is continuous w ithout intronThe cDNA of PeZFP gene contains an open read ing fram e of 495 bp and codes for a protein of 164 aa(GenB ank accession number AC 01101) The database search using the am ino acid sequence as query show ed high hom ology to several zincfinger proteins, especially the PeZFP sequence show ed them axmi um hom ology( 877% identity) to OSISAP1 gene(O ryza sativa subs ecies indica stressassociated rotein g ene ), and it has a ty ically conserved sequence Cx 24Cx 9 12Cx 2Cx 4Cx 2Hx 5HxC (X re resents any am ino acids) at the Cterm inal AN 1ty e z inc f inger and the N term inal A 20 ty e z inc f ing erTheref ore, it red icted that the P eZFP