灯盏花素抑制脓毒症性急性肾损伤的机制的研究.pdfVIP

Med J Chin PLA, Vol. 39, No. 11, November 1, 2014 　849 论　著 ·基础研究· 灯盏花素抑制脓毒症性急性肾损伤的机制研究 杨梅，张岩，林锡芳，张艳杰，王丹，王晓蓉，高秋琦，李卓英，郑少玲 [摘要]　目的　观察灯盏花素对脓毒症大鼠急性肾损伤(A KI ) 的保护作用，并初步探讨相关机制。方法　36 只 Wistar雄性大鼠随机均分为对照组、脓毒症模型组(模型组)和灯盏花素治疗组(治疗组) ，每组12只。采用盲肠结扎穿孔 术复制脓毒症模型。术后24h处死大鼠，检测大鼠血肌酐(Scr) 、尿素氮(BUN) 、内皮素- 1(ET- 1) 、诱导型一氧化氮合酶 (iNOS)水平，肾组织超氧化物歧化酶(SOD)活性、脂质过氧化产物丙二醛(MDA )及iNOS蛋白含量，并取肾脏组织作病 理组织等分析。结果　与对照组比较，模型组大鼠血清Scr 、BUN 、ET- 1及iN OS水平显著升高，肾组织SOD活性明显 降低，MDA及iNOS蛋白含量显著升高(P 0.05) 。与模型组比较，治疗组大鼠血清Scr 、BUN 、ET- 1及iNOS水平明显降 低，肾组织SOD活性明显升高，MDA及iNOS蛋白含量显著降低(P 0.05) 。结论　灯盏花素对脓毒症大鼠A KI具有明显 保护作用，其机制可能与改善肾脏微循环及提高抗氧化酶活性、降低氧化产物含量有关。 [关键词]　脓毒症；肾功能衰竭；灯盏花素；氧化还原酶类 [中图分类号]　R692.5　　　　[文献标志码]　　　　　[文章编号]　0577-7402 (20 14)11-0849-05 [D OI]　10.11855/j.issn.0577-7402.20 14.11.0 1 Mechanism of the preventive effect of breviscapus on pathogenesis of acute kidney injury in rats with sepsis YANG Mei, ZHANG Yan, LIN Xi-Fang, ZHANG Yan-jie, WANG Dan, WANG Xiao-rong, GAO Qiu-qi, LI Zhuo-ying, * ZHENG Shao-ling Intensive Care Unit, First Affiliated Hospital of Wenzhou Medicine University, Wenzhou, Zhejiang 325000, China * Corresponding author, E-mail: zsl@ This work was supported by the Science and Technology Project of Wenzhou ( [Ab stract]　Objective　To investigate the inhibitory effect and its related mechanism of Erigeron breviscapus on pathogenesis of acute kidney injury (AKI) in rats with sepsis. Methods　Thirty-six male Wistar rats were randomly assigned into control group, sepsis model group (CLP group), and breviscapine treatment group (treatment group, n=12 each). The sepsis model was reproduced by cecal ligation and puncture (CLP). The rats were sacrificed 24 h after operation. The levels of blood urea nitro