一株解磷细菌的筛选、鉴定及溶磷培养条件的优化.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 2 :173-178 一株解磷细菌的筛选、鉴定及其溶磷培养条件的优化 黄达明  李倩  管国强  张志才  钱静亚  宋庆春 （江苏大学食品与生物工程学院，镇江 212013） 摘 要 ： 从土壤作物根际筛选分离出的一株解磷能力较强的溶磷菌P0417，对其进行16S rDNA 基因水平上的初步鉴定，测 定其溶解磷的能力，并对该菌的溶磷培养基条件进行优化。结果表明，经序列分析，确定该菌株P0417 为洋葱伯克霍尔德氏菌。且 其溶磷能力与培养液pH 呈显著相关性，当培养基条件为葡萄糖10 g/L、草酸铵0.5 g/L、NaCl 1.0 g/L 时，菌株P0417 对Ca （PO ） 3 4 2 盐培养基具有较好的解磷能力，其解磷能力可达791.84 μg/mL。 关键词 ： 溶磷细菌 ；筛选；鉴定 ；条件优化 ；解磷能力 DOI ：10.13560/ki.biotech.bull.1985.2015.02.026 - ， Selection Identification and Medium Optimization of a Phosphate solubilizing Bacterium Huang Daming Li Qian Guan Guoqiang Zhang Zhicai Qian Jingya Song Qingchun （ ， ， 212013 ） College of Food and Biology Engineering Jiangsu University Zhenjiang Abstract ： A phosphorus-dissolving bacterial strain P0417 was isolated from crop rhizosphere soil and identified by the genetic identification of 16S rDNA, measuring capability of phosphate-solubilization and optimization of medium components here. The result demonstrated that the bacterium was identified as Burkholderia cepacia. And the capacity of dissolving the phosphate was closely correlated with the pH of its culture medium, the strain showed high phosphate-dissolving ability of 791.84 μg/mL in Ca （PO ） culture medium at glucose, 10 3 4 2 g/L ；Ammonium ox