小鼠脂肪细胞苏丹红染色.doc

Cell biology?experiments?report Topic：Sudan Red?staining?of?fat cells?in?mice Name: Crew: Tutor: Student ID: Time： Digest： Cells are surrounded by a membrane, independent breeding the smallest group of protoplasm, while the cells are the basic structural and functional units of the activities of life, the cell composition of water, so the cells or organelles under the microscope is a colorless, hard to tell?boundaries between cells, in normal circumstances by staining to distinguish cells, staining, usually by some special parts of the cell, or special component into chromatin, and thereby to distinguish from the tissue cells. In adipose tissue, lipid substances can be stored in the form of oil particles in the fat cells, but in the low magnification under the microscope, the granular material is difficult to see, unless the stain, thus observed.?Commonly used to stain the dye Sudan fluid, such as Sudan III, Sudan V. Sudan Ⅲ is a common right triglycerides were stained fat soluble dye.?Its color is reddish brown, the maximum UV absorption of 507 nm, at (304).?Sometimes also known as Sudan Red BK, Fat Ponceau G, Cerasin Red, CI 26100, Solvent Red 23, Sudan Red, Sudan Red III, Sudan V, Sudan Red B, Sudan G, Scarlet B, and Tony Red..?In industry, it is often used to non-polar substances, like oils, fats, waxes, animal fats and oils and other hydrocarbon products, and acrylic emulsion, or emulsion. Ⅰ, Sudan, Sudan III, Sudan IV has been the International Association for Cancer Research go to the three types of carcinogens, great harm on the human body. Fat cells to store fat, and adipose tissue by fat cells, which store energy.?Mainly by two kinds of adipose tissue, white adipose tissue (WAT) and brown adipose tissue (BAT), which is composed of two different kinds of fat cells in the mouse mesentery is the WAT.????Sudan dyes solubility in lipids is greater than its solubility in solvents, dyes and samples containing lipids in contact