人工复制小鼠铜离子中毒的病理变化观察 毕业设计.docxVIP

PAGE  PAGE 12 毕业设计（论文） 题　　目：人工复制小鼠铜离子中毒的病理变化观察 系　　部： 动科系　　　　　 专　　业：　　　　　　　　　　　　 学　　号：　　　　　　　　　　　　 学生姓名：　　　　　　　　　　　　 导师姓名：　　　　　　　　　　　　 导师职称：　　　　　　　　　　　　 二○一三年四月（填写时间要用中文） 内容摘要及关键词 【摘 要】：本研究采用硫酸铜灌胃的方式对小鼠铜中毒的临床表现及组织器官的病理变化进行观察。试验根据灌胃的剂量将实验小鼠随机分为 4 组：对照组 (0 mg/kg)、低剂量组 (50mg/kg)、中剂量组 (100mg/kg)，高剂量组 (200mg/kg)，每组 6 只，试验周期为 2 周。结果显示，高剂量组的死亡率高于中剂量和低剂量组。高剂量组器官的病理形态学观察表现为肝脂肪变性和颗粒变性，肝细胞呈现蜂窝状外观，低剂量组和中剂量组肝细胞肿大，胞浆内充满红染的细微颗粒；低剂量组中肾小管出现颗粒变性，肾小管上皮细胞肿胀，使管腔变小或狭窄，间质毛细血管扩张，充血。中剂量组肾小管出现明显颗粒变性，管腔中有许多红染蛋白质物质，出现管型。部分肾小管壁细胞结构被破坏。高剂量组肾小管上皮坏死、崩解、脱落于管腔中。结果表明，铜中毒主要损害肝脏、肾脏等组织器官，最终导致发病甚至死亡。 关键词：铜中毒；病理变化；小白鼠； Abstract and keywords [Abstract]: the pathological changes in this study by copper sulfate gavage to mice copper poisoning clinical manifestations and organs were observed. According to the gavage dose mice were randomly divided into 4 groups: control group (0 mg/kg), low dose group (50mg/kg), middle dose group (100mg/kg), high dose group (200mg/kg), 6 rats in each group, the test cycle for 2 weeks. The results showed that, the high dose group was lower than that in middle dose and low dose group. Pathological observation of the high dose group of organs showed degeneration of liver steatosis and particles, liver cell showing a honeycomb appearance, low dose group and middle dose group liver cell swelling, fine granular cytoplasm filled with red dye; low dose group of renal tubule showed granular degeneration, swelling of renal tubular epithelial cells, so that the lumen or stenosis, interstitial capillary dilatation, hyperemia. The middle dose group showed granular degeneration of renal tubular lumen, there are many red dye protein substances, the tube type. Structure of cell wall tubular damage. High dose group renal tubular necrosis, disintegration, shed from the lumen. The results show that copper poisoning, major damage to the liver, the kidney, eventually lead to disease or death.关键词：铜中毒；病理变化；小白鼠； Keywords: copper poisoning， pathological changes， rat TOC \o 1-3 \