生物预处理秸秆转化能源的初步研究 毕业论文.doc

生物预处理秸秆转化能源的初步研究 A Preliminary Study of The Biological Pretreatment Straw to Energy  摘 要 白腐菌是目前已知的降解木质素最强的微生物，但白腐菌直接对秸秆进行预处理所得到的糖化率和乙醇的产量并不高。国外研究显示，利用白腐菌对秸秆等草类原料进行固体发酵处理，可以对木质素进行选择性降解，直接攻击胞间层中的木质素，导致纤维的分离。目前，国内利用白腐菌的生物降解研究大都处于实验室阶段。 前期研究通过对自然界微生物天然腐朽过程的模拟和改造，建立了一个可用于秸秆转化乙醇、油脂等生物能源的仿生预处理系统，该系统的处理效率是目前同类报道中最高的。对这一系统优化增强系统稳定性，为其工业化奠定基础。K+、Mn、Ca、Cu5个优化的因子,即：在28℃温度条件下培养4周、通氧并添加低浓度Mn2+离子，提供更高的培养基湿度，可以提高白腐菌预处理秸秆转化能源的效率。 关键词：白腐菌 秸秆预处理 优化 糖化率 Abstract Because of reduced energy and less pollution, biological pretreatment attracts a wide spread attention.The biological degradation of lignin in corn straw with white rot fungi is the most effective fungi among Microbe.Overseas studies have shown that white rot fungi the solid fermentation processing the raw material of straw and other grasses, selective degradation of lignin, a direct attack on the lignin in the cell between layers, resulting in separation of the fiber.Currently,the domestic use of white rot fungus biological degradation studies mostly in the laboratory stage. Preliminary studies by the nature of microbial natural decay process simulation and transformation, the establishment of a bionic pretreatment system for straw into ethanol, oil and other bio-energy, the processing efficiency of the system is similar reported. Pretreatment of straw from the saccharification rate in order to significantly improve the white rot fungus, the white rot fungus pretreatment straw system conditions optimized to enhance system stability and lay the foundation for its industrial application. The research topics include: biological pretreatment under open conditions; the biological pretreatment system stability; major factor affecting biological pretreatment stability.To improve the saccharification distinctly, we researched the influence of different culturing conditions of white rot fungus on the pretreatment of corn.We designed different culturing temperature and time and to