2干化学讲座课件.docVIP

Dry Chemistry 干化学  Variable Dry Chemistry 干化学之种类 ? Baxter Paramax, Dupont Dimension Ames z Multi-Film 多层薄膜 ? Ortho Vitros System, FujiChem    Indicator layer 指示剂层 Support layer 支持层  ? Uniformly distributes the sample   Uniformly distribute the sample 均匀分布标本      均匀分布标本 z Filters out large molecules 可包含打断大分子的试剂：物理性和 化学性 z A background for reflective purpose 提供反射测定的背景  A Background for Light Reflection 光反射的背景  ? Contains reagent in dry form Support layer 支持层  ? Drive reaction toward ? Contains a dye to produce a colored complex Indicator layer 指示层 Support layer 支持层  Support Layer 支持层 ? Provide for a supporting ? Allows lights to pass through Indicator layer 指示层 Support layer 支持层  Transmission Reflection 透射与反射  Transmission Reflection 透射与反射 BUN Reaction Result  比色/ 速率干片  Measure chemistry concentration by color development 通过颜色的变化来测定化学物浓度  ? Potassium (K+)钾离子 ? Measures the potential difference between the two electrodes 测定两电极间电势的不同   Chemical Potential = ? i = ? 0 + RT In ai  V One Dry ISE Slide Paper in plastic = salt bridge V  ? Examples of IR tests:免疫速率法干片举例： ? Digoxin, Phenytoin, Phenobarbital, CRP ?地高辛，苯妥英，苯巴比妥，C－反应蛋白 ? Competitive Non-competitive IR format 竞争与非竞争性免疫速率反应模式 ? Liquid substrate is required 需要液体反应底物  Spreading Layer 分布层 Receptor Layer 受体层 Gelatin Layer 胶乳层 Enzyme Antibody Enzyme-labeled Antigen   Patient Sample 病人标本 Enzyme Labeled Hapten 酶标  Patient Sample Enzyme Labled Hapten Immobilized Ab  Enzyme Labled Hapten + Antigen in Sample     Determine Analyte Conc. 分析物测定 (Competitive Method竞争性方法) Low Conc. High Conc.