EffectsofdifferentdosesofXrayontelomeraseinhumanosteosarcomacells.docVIP

Effects of different doses of X-ray on telomerase in human osteosarcoma cells (HOS cells) Lijun WANG 1 Honggang QIAN 2 Ming LI 1 Yuxing SHAN * Department of Orthopedics Norman Bethune 1st Hospital of Jilin University1* Department of Orthopedics the People’s Hospital of Jilin Province2 Changchun, China e-mail:yuxingshan@ *Corresponding author Haiyu ZHANG * Guolong SUN 3 Department of Children’s Surgery Norman Bethune 1st Hospital of Jilin University* Department of Thoracic surgery Norman Bethune 1st Hospital of Jilin University3 Changchun, China e-mail:zhanghaiyu1622@ Abstract—To investigate the effects of different doses of X-ray on the telomerase in human osteosarcoma (HOS) cells and to provide theoretical evidence for the clinical application of radiotherapy in osteosarcoma. Log phase HOS cells were radiated using 1, 2, 4 and 8 Gy doses of X-ray separately and cell survival was measured using MTT method, telomerase activity by ELISA, and telomerase reverse transcriptase (hTERT) expression by immuno-histochemistry. Telomerase RNA was also quantitatively analyzed using RT-PCR. Cell apoptosis was positively correlated to X-ray doses and telomerase activity was enhanced greatly, as well as hTERT and telomerase RNA expression. Under the interference of X-ray, X-ray doses were negatively correlated to HOS cell survival and positively correlated to telomerase activity; HOS cell survival was negatively correlated to telomerase activity; hTERT, telomerase RNA and telomerase activity changed synchronically. Key words-X-ray; human osteosarcoma cell; telomerase activity; hTERT; telomerase RNA Introduction Osteosarcoma is the most common malignant bone tumor. According to the WHO, it has a high incidence and accounts for 22.36% in primary malignant bone tumors. Typical osteosarcoma is usually seen in the 10-20 age-group, but it can occur in any age, including infants, children and elderly people. It is more common in males, the ratio of male: female is 2:1. It is hig