糖基转移酶与糖苷酶分析报告.ppt

第二节糖基转移酶及其应用 主要内容 糖转移酶简介 糖基转移酶在合成中的应用 Glycosyltransferases 应用‘activated’ sugar phosphates 作为糖供体，合成 glycosidic linkage ，糖受体通常为 nucleophilic group, usually an alcohol. 生成的糖苷可以为 O-, N-, S-, or C-glycoside 催化机理 Glycosyltransferases catalyze the transfer of glycosyl groups to a nucleophilic acceptor with either retention or inversion of configuration at the anomeric centre. This allows the classification of glycosyltransferases as either retaining or inverting enzymes. 糖基转移酶辅因子 Many, but not all, glycosyltransferases utilize divalent metal ion cofactors such as Mn2+ and Mg2+. … mainly in