荞麦中的多酚提取和鉴定预案.doc

本科毕业论文（设计） 姓 名 顾焱 班 级 应用化学1101 学 号 4103110124 指导老师 刘琴 答辩日期 2015.06.06 目录 1前言 6 1.1荞麦简介 6 1.2植物多酚 6 1.3植物多酚的生物活性 7 1.3.1清除自由基、抗氧化 7 1.3.2抗病毒、抗菌功能 7 1.3.3抗肿瘤活性 7 1.3.4抗心脑血管疾病 8 1.3.5美白防晒、延缓衰老 8 1.4甜荞多酚 8 1.5多酚的提取方法 8 1.6多酚的分离纯化方法 9 1.6.1大孔树脂柱层析 9 1.6.2聚酰胺柱层析 9 1.6.3葡聚糖凝胶柱层析 10 1.6.4 HPLC/MS检测方法简介 10 1.7多酚抗氧化活性的测定方法 11 1.7.1 DPPH法(Radical Scavenging Capacity Assay) 11 1.7.2 FRAP法(Ferric-reducing Antioxidant Power) 11 1.8本文研究目的 12 2实验材料与方法 13 2.1 材料和试剂 13 2.2 仪器设备 13 2.3 实验方法 14 2.3.1样品的预处理 14 2.3.2荞麦麸皮中多酚的提取 14 2.3.3液质联用法(HPLC-MS)鉴定甜荞麸皮中的多酚 14 2.3.4甜荞麸皮多酚的分离纯化 15 2.3.4.1大孔树脂分离 15 2.3.4.2聚酰胺分离 16 2.3.4.3 Sephadex-LH 20葡聚糖分离 16 2.3.5分离纯化产物的抗氧化性分析 17 2.3.5.1 DPPH法 17 2.3.5.2 FRAP法 18 2.3.6数据分析 20 3实验结果与讨论 20 3.1荞麦麸皮粗提液的液相色谱析 20 3.2大孔树脂分离纯化 22 3.3聚酰胺分离纯化 23 3.4 Sephadex-LH 20葡聚糖的分离纯化 26 3.5 咖啡酰吡喃鼠李糖苷-吡喃葡萄糖苷的抗氧化性分析 27 4结论与展望 29 5参考文献 29 6致谢 32 甜荞麸皮中咖啡酰吡喃鼠李糖基-吡喃葡萄糖苷的分离纯化及抗氧化活性分析 摘要：本文主要以为原料，，对中的基-吡喃葡萄糖苷进行分离纯化基-吡喃葡萄糖苷基-吡喃葡萄糖苷 关键词：甜荞麸； 多酚； 分离纯化； 咖啡酰吡喃鼠李糖基-吡喃葡萄糖苷；抗氧化性 The purification and antioxidant activity studies of caffeoyl-rhamnopyranoside-glucopyra-noside in common buckwheat bran Abstract:In this work, polyphenol in common buckwheat bran was separated and purified by using column chromatography in order to get caffeoyl-rhamno-pyranoside-glucopyra-noside. The antioxidant activity of purified caffeoyl-rham- nopyranoside-glucopyra-noside was studied as well. Fifteen polyphenol species were detected in the common buckwheat bran, and caffeoyl-rhamnopyranoside- glucopyra-noside had highest content. Therefore, caffeoyl-rhamnopyranoside-glu- copyra-noside was purified by colomn chromatography method and macroporousresin, polyamide combine with glucan (Sephadex-LH20) ere used as purification ma- terial. The antioxidant activities of purified product were then studied by DPPHand FRAP method. The results showed that the antioxidant activity of caffeoyl-rha