细胞发展剖析.ppt

刘金涛 E-mail:jtliu0416@ 华东理工大学海正研究院 动物细胞与组织工程研究所 Glucose 对于连续细胞系，丙酮酸脱氢酶、磷酸烯醇式丙酮酸羧化酶和丙酮酸羧化酶它们活性很低，导致糖酵解生成的丙酮酸很难进入TCA循环。 Glucose is used in most formulations to provide an energy source as well as a precursor for biosynthesis. Lactic acid is the major product of glycolysis. In most cultures only a small proportion of glucose is completely oxidized via the tricarboxylic acid (TCA) cycle. Glutamine L-Glutamine is important as a precursor for Peptide and protein synthesis amino sugar synthesis, Purine，pyrimidine ，nucleic acid and nucleotide synthesis providing a source of carbons for oxidation