《The Literature Report》.ppt

TLC：Rapid, Robust, and Cost-effective High sample throughput, accessibility for postchromatographic evaluation, and requirement for minimal sample cleanup. Used for large scale surveillance programs, class fractionation and species separation of lipids, and the analysis of complex and “dirty” samples. MS：High sensitivity, high specificity Rhodamine B, fluorescein, Sudan III, and methyl violet Chloramphenicol, nateglinide, and gliclazide Quinine A longjing tea sample Methanol of HPLC grade purified water Cellulose-coated TLC glass sheets (thickness, 0.25 mm, with fluorescent indicator, G/UV 254) silica gel-coated TLC glass sheets (thickness, 0.2 mm, with fluorescent indicator, G/UV 254) Longjing Tea Sample: 1g of ground Longjing tea leaves was ultrasonically extracted for 20min with 10mL of methanol/H2O (70/30, v/v). Then, the extract was filtered, concentrated, and developed by normal phase silica gel-coated TLC. Standard Solutions: Dye mixtures in the methanol were 6.3mmol/L rhodamine B + 6.0 mmol/L fluorescein+8.5mmol/L Sudan III Drugs in methanol were 9.3mmol/L quinine+9.3mmol/L gliclazide+9.3 mmol/L chloramphenicol Optimization of TLC/PAMLDI-MS were all prepared at a concentration of 6mg/mL. TLC Separation: For the separation of dyes, the developing agent was dichloromethane/ethanol/ammonia (66.1:33.1:0.8, v/v/v) For the separation of drugs, the developing agent was hexane/ethanol/formic acid (64.7:32.4:2.9, v/v/v) For the tea extract, hexane/ethanol (1:2, v/v) Plasma assisted multiwavelength laser desorption ionization mass spectrometry (PAMLDI-MS), was successfully coupled with TLC for rapid analysis of complex samples.The three-wavelength laser system provides great advantages in detecting different species of low molecular weight compounds.Detailed interpretations of the mass spectra obtained from IR, visible, and UV-PAMLDI-MS were discussed, which indicated that higher energy photons, like UV photons, resulted in a more severe extent of cleavage of bonds