《Development of glucose biosensors based on nanostructured graphene-conducting polyaniline composite》.ppt

东华理工大学化学生物与材料科学学院 Faculty of Biology, Chemistry and Material Science, East China University of Technology Reporter： SunWenxi Date：2015.08.27 Literature Reports Development of glucose biosensors based on nanostructured graphene-conducting polyaniline composite Xue Feng, Huijun Cheng, Yiwen Pan, Hao Zheng Institute of Marine Chemistry and Environment, Ocean College, Zhejiang University, Hangzhou 310027, PR China Content 1. Introduction 2. Material and methods 3. Results and discussion 4. Conclusion 1.Introduction The meaning of development of glucose biosensors A healthy problem -----Diabetes a novel glucose based on GRA- PANI nanostructured composite have been proposed 2. Material and methods Reagents and materials 2.1 Instrumental 2.2 Preparation of GRA–PANI modified electrodes 2.3 Fabrication of the amperometric glucose biosensors 2.4 Electrochemical measurements 2.5 PPT模板下载：/moban/ 2.1. Reagents and materials Glucose oxidase (GOD, BC grade) from Aspergillus niger D-galactose, glycine, L-tyrosine, L-phenylalanine, urea, aniline chitosan (CS, deacetylationZ95%) Glucose, ascor-bic acid, sodium phosphate monobasic dihydrate and sodium phosphate dibasic dodecahydrate . All other reagents and chemicals were of analytical grade and were used as received without further purification. 2.2. Instrumental The cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) amperometric measurements were per-formed using a PARSTAT 4000 electrochemical workstation A typical three-electrode system was used in this work with saturated calomel electrode (SCE) as the reference electrode, platinum (Pt) disk electrode with a diameter of 2 mm as the counter electrode and modified Pt disk electrode as the working electrode. Scanning electron microscopic images were obtained by SU1510 scanning electron spectroscopy (SEM) (Hitachi). 2.3Preparation of GRA–