微生物,韩超、李文亚、杨文飞.资料.ppt

Optimization of Microwave-Assisted Extraction of Polyphenols from Herbal Teas and Evaluation of Their in Vitro Hypochlorous Acid Scavenging Activity Journal of Agricultural and Food Chemistry 中华茶文化 中华茶文化 中国十大名茶：安溪铁观音、西湖龙井、太湖碧螺春、黄山毛峰、六安瓜片、信阳毛尖、太平猴魁、庐山云雾、蒙顶甘露、顾渚紫笋茶。 中华茶文化 Tea polyphenols 茶多酚 Hypochlorous acid (HOCl)次氯酸 人们已经意识到体内的ROS是导致众多疾病的罪魁祸首。作为氧化性物质的次氯酸是众多ROS的一种,机体内次氯酸(HClO)主要由髓过氧化物酶和血管过氧化物酶催化生成,HClO氧化性极强,基础水平的HClO在机体防御系统中起重要作用。然而,高浓度的HClO则给机体组织细胞造成氧化损伤。研究表明,HClO介导的氧化应激参与了动脉粥样硬化、细胞凋亡和衰老等多种病理生理过程。HClO可通过损害血管内皮功能、氧化修饰脂蛋白和增加斑块的不稳定性等多条途径促进动脉粥样硬化形成;通过影响线粒体渗透性转运孔和凋亡信号分子等途径促进细胞凋亡以及通过抑制抗衰老蛋白分子的表达和活性等途径促进细胞衰老。? 间苯二酚 间二苯酚因为它可以作为一种荧光物质而人们熟知，分子荧光系数ε = 1.59 × 107M?1cm?1，可以和次氯酸发生反应， 它的荧光密度可因次氯酸的氯化而降低。因此它可以作为一种荧光探针。 间苯二酚作为荧光探针 HOCl可以与间苯二酚探针反应使其氯化降低其荧光密度 多酚作为次氯酸的清除者可以阻止探针的氯化 RESULTS 1.Fluorescence intensity as a function of time 2.The fluorescence spectra of resorcinol with varying QR concentrations 3.Optimization of MAE Conditions 4.Testing of Incubation Reaction Products with the Resorcinol Assay 5.Analysis of the Results of Resorcinol and KI/Taurine Methods 6.Analysis of the Results Obtained by Resorcinol and HPLC Methods 7.Application of the Resorcinol Method to Some Herbal Extracts 1. Fluorescence intensity as a function of time 2.The fluorescence spectra of resorcinol withvarying QR concentrations 3.Optimization of MAE Conditions 4.Testing of Incubation Reaction Products with the Resorcinol Assay 5. Analysis of the Results of Resorcinol and KI/Taurine Methods 6. Analysis of the Results Obtained by Resorcinol and HPLC Methods 7. Application of the Resorcinol Method to Some Herbal Extracts DISCUSSION 1. The existing methods for quantifying HOCl scavenging activity have some limitations, such as possible UV absorption in the KI/taurine assay. The widely used TNB method, on the basis of the prevention of TNB oxidation in the presence of HOCl scavengers, may suffer from the extra capability of thiols to directly reduce 5,5 ′-dithiobis