RNAi沉默AEG1基因对人肾癌细胞生物学行为影响.pdf

万方数据 四、本研究创新性的自我评价···??············??·····?ぁ??材料与方法·····??????????????·．?实验结果···········??··?···??···················．?讨?睢??ぁ??ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぃ??前言··?ぁぁぁぁぁぁぁぁ?灰灰灰灰灰灰灰灰灰灰灰灰弧啊啊啊耙弧啊??讨?帧ぁぁ?ぁぁぁぁぁぁぁぁ??ぁぁぁぁぁぁぁぁぁ????ぁぁぁぁぁぁぁぁぁぁぁぁ??前言···············?ぁぁ??灰灰灰灰灰弧薄??????材料与方法?·?ぁぁぁぁぁ?ぁぁぁぁぁぁぁぁぁぁぁぁぁぁ?ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ??实验结果?·??一一一??一一一一一一一一一一一””一??五、参考文献·······?······················“”“一”””“·??目录实验结果··························???????···’?英文论著摘要·············································召材料与方法················································．?一、摘要中文论著摘要·············································?二、英文缩略语···············································??三、论文论文一前言·····················································??论文二材料与方法··········?·?ぁ?·······························．?实验结果··················································?讨?亍ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ??论文三前言·····················································?论文四讨?睢ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ?? 万方数据 综述···???·········································??个人简介··??·······································?电?在学期间科研成绩···········································啦?六、附录致谢·····················································?? 万方数据 沉默???基因对人肾癌细胞生物学行为的影响前言材料和方法·中文论著摘要·肾癌又称肾细胞癌，是起源于肾脏的肾小管或集合管的上皮细胞的恶性肿瘤，是泌尿系统中最常见的恶性肿瘤之一，其发病率仅次于膀胱肿瘤。目前，外和转移。此外，肾细胞癌对常规放化疗均不敏感。因此，研究肾细胞癌的发生机制，对于寻找肾细胞癌新的治疗靶点，开发更为有效的治疗手段，以延长患者的生存时间，具有重要的理论和实际意义。??．?珹???怯蒘?热?于??年利用快速抑制消减杂交技术在???或肿瘤坏死因子．口诱导的原代人胎脑星形胶质细胞中发现的。免疫组化和免疫荧光对???在细胞中的定位发现???主要定位于核周和类内质网区域。近年来研究证实，??猯在许多恶性肿瘤组织中高表达，并参与肿瘤进展的主要过程，包括细胞转化、细胞凋亡的抑制、侵袭、转移、血管新生和化疗药物抵抗等。此外，??等的研究表明???在大部分肾细胞癌组织中高表达，并且与肿瘤分化及进展有关。然而，???谏?细胞癌发生机制和化疗药物敏感性的作用仍不十分清楚，有待进一步研究。本研究通过构建???膕??干扰质粒，稳定转染体外培养的肾癌细胞株，探讨下调???的表达对肾癌细胞增殖、细胞周期、癌细胞凋亡和对????嘧啶敏感性的影响及相关生物学机制。这些结果表明沉默????蚩梢砸种葡?胞的增殖和集落形成，延缓及阻滞细胞周期于?／?期，最终促进肾癌细胞凋亡，作用并可能成为对肾细胞癌进行基因治疗的潜在靶点。科手术仍然是肾细胞癌治疗的主要方法，然而仍有约?％的患者手术后出现复发星形胶质细胞升高基因．??????并增加了化学药物敏感性。因此，???在肾细胞癌的形成和发展中发挥重要的???? 万方数据 一、细胞培养二、质粒构建三、细胞转染和稳转