非编码基因与肿瘤.ppt

;1. microRNA的研究历史 2. microRNA分布和作用机制 3. microRNA与癌症 4. microRNA研究的一般步骤和方法 5 以一篇文章为例介绍一下研究的过程;1993;1993;1993;1993;1993;1993;1993;1993;1993;1993;1993;1993;1993;1993;;;;2. microRNA分布和作用机制;Overall, 98 of 186 (52.5%) of miR genes are in cancer-associated genomic regions or in fragile sites;;;;;microrna与癌症;The let-7 family negatively regulates Ras;Ras家族有3个成员，即Nras、Kras、Hras分别含有9个、8个和3个let-7的靶位点，在HepG2细胞系中过表达let-7显著降低RAS蛋白的水平，在HeLa细胞系中转染let-7的反义抑制分子，则会提高RAS蛋白的水平;の;miR-17–92簇; ;与p53相关的miRNA;;;;;4. microRNA研究的一般步骤和方法;4. microRNA研究的一般步骤和方法;;Real-time PCR;Real-time PCR;Microarray;;;;;;;Nature 449, 682-688 (11 October 2007);;;;5.1 miR-10b? cell migration and invasion in vitro 5.2 miR-10b ? tumour invasion in vivo 5.3 miR-10b ? distant metastasis in vivo 5.4 how miR-10b expression is regulated? 5. 5 What is a direct and functional target of miR-10b? 5.6 miR-10b expression is elevated in metastatic breast tumours;5.1 miR-10b? cell migration and invasion in vitro;Although neither of these two antisense RNAs affected the motility of MDA-MB-231 cells;Trypan blue exclusion assay(台盼蓝拒染法 );miR-10b overexpression;These results indicated that overexpression of miR-10b is sufficient to promote both migration and invasion in vitro.;5.1 miR-10b? cell migration and invasion in vitro 5.2 miR-10b ? tumour invasion in vivo 5.3 miR-10b ? distant metastasis in vivo 5.4 how miR-10b expression is regulated? 5. 5 What is a direct and functional target of miR-10b? 5.6 miR-10b expression is elevated in metastatic breast tumours;5.2 miR-10b ? tumour invasion in vivo;;;;5.1 miR-10b? cell migration and invasion in vitro 5.2 miR-10b ? tumour invasion in vivo 5.3 miR-10b ? distant metastasis in vivo 5.4 how miR-10b expression is regulated? 5.5 What is a direct and functional target of miR-10b? 5.6 miR-10b expression is elevated in metastatic breast tumours;5.3 miR-10b ? distant metastasis in vivo;;;;SUM159+ miR-10b;;5.1 miR-10b? cell migration and invasion in vitro 5.2