smad4在结肠癌组织中的表达及其临床意义.docVIP

Smad4在结肠癌组织中的表达及其临床意义 刘岩青1，盛吉章2，赵磊2，杨猛1，杨文华1 戴殿禄1 1. 沧州市中心医院肿瘤外二科 河北省沧州市 061000 2. 沧州市中心医院心内科 河北省沧州市 061000 刘岩青，女，博士学位，主治医师，主要从事消化系统肿瘤的临床与基础研究. E-mail：liuyanqing927@163.com 通讯作者：戴殿禄，男，主任医师，研究方向：消化系统肿瘤的临床与基础研究. E-mail：daidianlu111@163.com 摘 要 目的：探讨Smad4 mRNA的表达在结肠癌组织与癌旁正常组织之间的表达差异，及与结肠癌临床病理参数与预后的关系。 方法：选择78例结肠癌手术切除的标本，选取结肠癌组织和相应的癌旁正常组织；采用荧光定量逆转录聚合酶链反应（RT-PCR）检测两组Smad4 mRNA表达水平，Kaplan-Meier法和Cox比例回归模型分析Smad4 mRNA表达与结肠癌临床病理参数与预后之间的关系。 结果：结肠癌组织中Smad4 mRNA表达水平显著低于癌旁组织(P0.05)。淋巴结转移、血管淋巴管浸润、肿瘤TNM分期与Smad4-mRNA负相关(P0.05)。Kaplan-meier生存分析显示Smad4高表达的结肠癌患者有更长的生存期。多因素分析结果显示Smad4 mRNA表达水平是影响结肠癌预后的独立影响因子。 结论：Smad4表达在结肠癌的发生、发展发挥着抑制因子的作用，是影响结肠癌预后的独立影响因子。 关键词：结肠癌；Smad4；RT-PCR；预后 The correlation of Smad4 expression with clinical characteristics and its relevance to prognosis in colorectal carcinoma Yan-Qing Liu1, Ji-Zhang Sheng2, Meng Yang1, Lei-Zhao2, Wen-Hua Yang1，Dian-Lu Dai1 Department of Oncology, Cang Zhou Central Hospital, CangZhou, 061001, Hebei Province. Department of Cardiology, Cang Zhou Central Hospital, CangZhou, 061001, Hebei Province. Correspondence to: Professor Dian-Lu Dai, Department of Oncology, Cang Zhou Central Hospital, CangZhou, 061001, Hebei Province, China. Daidianlu111@163.com Aim: The aim of the present study was to investigate the Smad4 mRNA expression in colon cancer tissues and adjacent non-cancerous tissues and its potential relevance to clinicopathological variables and prognosis. Methods: 78 cases of colorectal cancer samples including colon cancer tissues, paracancerous normal tissues were selected. Smad4 mRNA expression of the 2 groups were detected by real-time fluorescence quantitative (RT-PCR). Kaplan-Meier analysis and Cox proportional hazards regression models were used to investigate the correlation between Smad4 expression and prognosis of colon cancer patients. Results : The expression level of Smad4 mRNA in colon cancer tissues was significantly lower than that of the paracancerous tissues and normal tissues (P0.05). Our results indi