miRNA206对ERα阳性乳腺癌细胞他莫昔芬敏感性的影响.pdfVIP

摘要 摘 要 目的：探讨 mir-206 对乳腺癌细胞 MCF-7 中 ERα 表达水平以及他莫昔芬敏 感性的影响。 方法：将 mir-206 mimic、mir-206 inhibitor 及相应阴性对照（mir-206 mimic NC、mir-206 inhibitor NC）分别转染乳腺癌细胞 MCF-7 中，用 RT-PCR 方法检 测转染后各组乳腺癌细胞中 mir-206 和 ERα mRNA 的表达量的变化，用 5ug/ml 他莫昔芬处理 MCF-7 细胞 24 小时后，MTT 法检测不同转染组细胞增殖情况。 结果：与 mir-206 mimic 阴性对照组比较，mir-206 mimic 组中乳腺癌细胞 mir-206 表达升高，ERα mRNA 表达降低；与 mir-206 inhibitor 阴性对照组比较， mir-206 inhibitor 组中乳腺癌细胞 mir-206 表达降低，ERα mRNA 表达升高。在 他莫昔芬作用 24 小时后，mir-206 mimic 组的乳腺癌细胞增殖不明显，(t=2.252， P=0.0544)；mir-206 inhibitor 组的乳腺癌细胞增殖明显受到抑制，（t=4.856， P=0.0013）。 结论：下调 mir-206 表达可以增加 ERα 阳性乳腺癌细胞对他莫昔芬的敏感 性。 关键词：miRNA-206、ERα、乳腺癌、他莫昔芬 II 万方数据 Abstract ABSTRACT OBJECTIVE: To investigate the influence of mir-206 on the expression level of estrogen receptor α and the sensitivity of tamoxifen of ERα positive breast cancer cells. METHODS: Expression of mir-206 and ERα mRNA of breast cancer cells MCF-7 was quantitated by RT PCR; the change of expression of mir-206 and ERα mRNA was quantitated after MCF-7 cells were transfected by mir-206 mimic, inhibitor and corresponding negative controls for 24h; cell growth was measured by utilizing MTT after transfected MCF-7 cells were treated with 5ug/ml tamoxifen for 24h. RESULTS：Introduction to MCF-7 cells with mir-206 mimic decreased ERα mRNA expression but had no notably effect on cell growth with tamoxifen (t=2.252， P=0.0544), while introducti