LPS致营养不良性肺损伤小鼠肺泡液体清除影响.pdfVIP

目 录 一、摘要 中文论著摘要?????????????????．1 英文论著摘要???????．??????????2 二、英文缩略语??????????．???????．．4 三、论文 前言????????????????．．．???b一刚舌 ???????．．．??? 材料和方法??????????????????5 结果???????????????．．．．．．???7 讨论???????????????．．????．9 结论???????????????????．．10 四、创新性的自我评价???????．???．．．????．12 五、参考文献?????????????????．?13 六、附录 综述????????????．．．．．??????15 在学期间科研成绩???????????????．．20 致谢???????????????????．．21 个人简介???????????????．???22 ·中文论著摘要· LPS致营养不良急性肺损伤小鼠肺泡液体清除的影响 目 的 探讨脂多糖致营养不良急性肺损伤小鼠肺泡液体清除的影响。 方 法 雄性C57BL／6小鼠，随机分为空白对照组、营养不良组、正常LPS诱导组、营 养不良LPS诱导组，禁食不禁水120h制备营养不良小鼠模型，腹腔注射 LVS(8mg／kg)24h制备急性肺损伤小鼠模型，并应用尾静脉注射脂氧素A4对各组 进行干预，测定肺泡液体清除率(AFC)、肺干／湿(D／W)比值的变化，组织病 理学检查。 结 果 小鼠禁食120h较自由摄食水小鼠AFC及D／W比值明显下降，禁食120h并 予LPS诱导急性肺损伤的小鼠AFC及D／W比值较自由摄食予LPS诱导的急性肺 损伤小鼠明显下降；脂氧素A4对自由摄食的LPS诱导的急性肺损伤小鼠AFC及 D／W比值明显改善，但是对禁食120h并给予LPS诱导急性肺损伤的小鼠的AFC 及D／W比值无明显改善。 结论 营养不良能加重LPS诱导的急性肺损伤小鼠的损伤程度，降低肺泡液体清除 功能及药物治疗效果。 关键词 营养不良；脂多糖；急性肺损伤；肺泡液体清除率 ·英文论著摘要· The Influence of Alveolar Fluid Clearance in Lioop01)Lccaride．．induced Acute lunglysacarl(1 ung_- injury Mice with Malnutrition obj ective To study the Influence of Alveolar Fluid Clearance in Lipopolysaccaride-induced Acute lung injury Mice with Malnutrition． Methods Male C57BL／6 mouse were randomly into groups：blank control group， malnutrition group，normal LPS-induced group，malnutrition LPS-induced group．Mouse were allowed access to water not to food for 1 20 h to make malnutrition animal model．Intraperitoneal injection LPS with concentration of 8mg／kg for 24h to make acute lung injury animal model．Then use caudal vein injection lipoxin A4 to intervene．Determine the changes of Alveolar Fluid Clearance(AFC)and Dry／Wet(D／W) in lung tissue，complete the lung tissue in pathology． Results The results of AFC and D／W in malnutrition mouse werelower than normal ones．The results of AFC and D／W in mouse with malnutrition were lower than the ones free·-fed commally on LPS··induced Acute lung injury；The lipoxin A4 were obviously improved the results of AFC and D／W in free-·fed LPS·-induced