小麦叶片蛋白在盐胁迫下的探究要点.pptxVIP

L. Gao, X. Yan, X. Li , G. Guo, Y. Hu, W. Ma, Y. Yan Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE) College of Life Sciences, Capital Normal University, 100048 Beijing, China College of Resource Environment and Tourism, Capital Normal University, 100048 Beijing, China Centre for Comparative Genomics, Murdoch University, Western Australia Department of Agriculture Food, Australia Phytochemistry 10, July 2011 IF: 2.547 赵明星 2012年12月11日 Abstract Salt stress is a major abiotic stress that limits agricultural productivity in many regions of the world. To understand the molecular basis of the salt stress response in wheat (Triticum aestivum L.), a proteomic approach was used to identify the salt stress-responsive proteins in an elite Chinese wheat cultivar, Zhengmai 9023, which exhibits a high yield, superior gluten quality and better biotic resistance. Three-week-old seedlings were treated with NaCl of four different concentrations (1.0%, 1.5%, 2.0%, and 2.5%). The total proteins from the leaves of untreated and NaCl-treated plants were extracted and separated by two-dimensional difference gel electrophoresis (2D-DIGE). A total of 2358 protein spots were detected on the gels, among which 125 spots showed a significant change in protein abundance, and 83 differentially expressed spots were localised on preparative gels. Using Q-TOF mass spectrometry, 52 salt-responsive spots were identified, which were classified into six functional categories that included transport-associated proteins, detoxifying enzymes, ATP synthase, carbon metabolism, protein folding, and proteins with unknown biological functions. Of the 52 differentially expressed proteins, 26 were up-regulated, 21 were down-regulated, and five spots showed multi-expression patterns. In particular, some important proteins for salt tolerance were found to be up-regulated in Zhengmai 9023 under salt stress, such as H+-ATPases, glutathione S-transf