乙肝五项患者血浆HBVDNA水平与乙肝五项指标相关性分析.docVIP

乙肝患者血浆HBV-DNA水平与乙肝五项指标相关性分析 摘 要 目的：探讨乙肝病毒脱氧核糖核酸（HBV-DNA）水平与乙型肝炎免疫标记物（乙肝五项HBsAg、HBsAb、HBeAg、HBeAb、HBcAb）间的关系。 方法：选取587例乙肝患者的病历资料，分析患者的HBV-DNA水平与乙肝五项免疫标志物的表达关系。在乙肝患者的血浆标本中，用荧光定量PCR技术来检测HBV-DNA的含量，据含量高低的不同，分为103/ml，103~105/ml，105~107/ml，107/ml这四个等级；用酶联免疫ELISA法检测乙肝五项指标（乙肝两对半：HBsAg、HBsAb、HBeAg、HBeAb、HBcAb），按照阴性、阳性统计。 结果：乙肝患者血浆HBV-DNA检出率为61.9%，HBsAg、HBsAb、HBeAg、HBeAb、HBcAb的检出率分别为98.45%、1.13%、42.68%、40.44%、96.26%。在HBV-DNA含量不同的乙肝患者中，五项的检出率也不尽相同。随HBV-DNA含量的提升，HBsAg、HBcAb的检出率没有出现明显的变化，同时HBeAg的检出率呈逐渐升高的趋势，HBeAb的检出率呈逐渐下降的趋势。 结论：乙肝患者血浆HBV-DNA水平与乙肝五项指标间存有密切的关系，随HBV-DNA水平的升高，乙肝五项免疫标志物会出现不同的表达，这对帮助医生精准地判断患者病情和实现快速对症治疗具有重要价值。 关键词：乙型肝炎，HBV-DNA，乙肝五项指标，酶联免疫法 HEPATITIS B PATIENTS WITH HBV-DNA LEVELS HBV FIVE INDICATORS CORRELATION ANALYSIS ABSTRACT Objective: to study the correlation between HBV-DNA levels and hepatitis B virus immunological-markers(HBV five indicators). Methods: medical records selected Affiliated Hospital of Zhengzhou University, Luoyang Center 587 cases of hepatitis B patients. In total,333 men patients with HBV were included;254 female patients with HBV infection were as followed. To investigate the clinical characteristics of hepatitis B surface antigen (HBsAg).seroclearance in patients with hepatitis B virus (HBV) infection. Analyses were made between HBV-DNA levels and hepatitis B five markers which were determined using fluorescent quantitative PCR and enzyme-linked immunosorbent assay. Respctively, the amount of HBV-DNA in patients with hepatitis B were detected by fluorescent quantitative PCR, according to the level of the content, divided into 103/ml,103~105/ml,105~107/ml,107/ml. Hepatitis B two pairs of semi-detection (HBsAg、HBsAb、HBeAg、HBeAb、HBcAb) were used by enzyme-linked immunosorbent assay,according to the statistics of positive or negative.Ratios of HBsAg to HBV-DNA seroclearance in serum were significantly different between patients men and women, Results: in these patients,the HBV-DNA positivity rate was 61.9%,the positivity