【精品医学论文】川芎嗪对大鼠脑缺血再灌注后梗死面积及ED1、IL-1和TNF-表达的影响.docVIP

川芎嗪对大鼠脑缺血再灌注后梗死面积及ED1、IL-1β和TNF-α表达的影响仅荼醴磨忆蒽卢鳖鹋冬庳汆罅痛【摘要】? 目的 通过观察川芎嗪对大鼠脑缺血再灌注后梗死面积及缺血脑组织单克隆抗体(ED1)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α) 表达的影响,探讨川芎嗪的作用机制。方法 将60只健康成年SD雄性大鼠随机分为假手术组、模型组及川芎嗪大、中、小剂量组,采用改良线拴法复制缺血再灌注脑梗死动物模型。川芎嗪大剂量组给予140 mg/kg川芎嗪静脉注射,中剂量组给予120 mg/kg静脉注射,小剂量组给予100 mg/kg静脉注射。实验结束后处死大鼠,从上述各组中取6只大鼠的脑组织取材制片、TTC染色、福尔马林固定后,采用病理图像分析系统,评价川芎嗪是否能减少脑梗死面积;将各组另6只大鼠断头取脑制片后,用免疫组化染色,观察ED1、IL-1β和TNF-α的阳性表达。结果 川芎嗪各治疗组可减少缺血90 min、再灌注24 h脑梗死面积,也可以减少脑梗死区域内的ED1、IL-1β和TNF-α的免疫阳性表达。结论 川芎嗪可以减少大鼠脑缺血再灌注后梗死面积,其治疗效用可能与抑制小胶质细胞活化和IL-1β、TNF-α的免疫阳性表达有关。 现峻愁吮颊蛄臻尾烈瓴规瀚杀菡 【关键词】? 川芎嗪;脑缺血再灌注损伤;单克隆抗体;白细胞介素-1β;肿瘤坏死因子-α;大鼠哑谅都财貉珈形芹藉谯身终伽粹 　　Abstract：Objective To investigate the effect of Tetramethyl Pyrazine (TMP) for treating cerebral infarct by observing infarct areas, and to research its function mechanism by observing the changes of the expressions of ED1, TNF-α and IL-1β in the infarction region after focal cerebral ischemia reperfusion injury. Methods Sixty SD male rats in healthy condition were randomly and averagely divided into normal group, model group and TMP high, middle and low dose group. The cerebral infarct animal model was reproduced by modified thread-tie method. TMP high dose group was administered TMP 140 mg/kg, middle dose group was 120 mg/kg and low dose group was 100 mg/kg by intravenous. Then all the rats were killed. Thirty rats were taken to evaluate whether TMP can decrease the ratio of cerebral infarction areas in the TTC-reacting and formalin fixation brain tissue slices taken from the above-mentioned killed rats, with Pathology Image Analysis System. The masculine of ED1, IL-1β and TNF-α in the immuno- reacting cells in cerebral infarction brain tissue slice taken from other thirty killed rats were observed. Results TMP groups decreased the ratio of cerebral infarction areas, and also decreased ED1, TNF-α and IL-1β in immuno-reacting cell. Conclusion TMP can decrease the formation of cerebral infarction caused by cerebral ischemia reperfusion and its effect is possibly related