小麦慢白粉、慢条锈抗性QTL的分析.pdfVIP

Abstract Powdery mildew and stripe 111st,caused by Blumeria graminis f．sp．tritici and Puccinia striiformis f．sp．t／rt／ci，respectively，are the most damaging diseases in comrl”li wheat(Triticum aestivum U． Utilization of resistant cultivars is all effective way to control the disease．Identification of powdery mildew and stripe rust resistance genes is essential for breeding resistant wheat cultivars．This study included quantitative trait loci mapping for adult-plant resistance to powdery mildew and analysis of slow stripe rust resistance in comllgm wheat． In the present study,a total of 432 molecular maxkers w∽used to map QTL for adult-plant resistance(APR)to powdery mildew in a doubled haploid(DH)population with 107 lines derived from the c“，ss Fukuho-komugi／Oligoculm．Held trials were conducted in Beijing and Anyaug during 2003-04 and 2004-05 cropping 8P∞lsous．respectively．The DH lines were planted in a randomized complete block design wi山three replicates．A_砌cial inoculation was calrled 0ll￡jn Bering witll highly virulent isolate E20 of Blumeria graminis￡sp．tritici and the powdery mildew severity on penultimam leaf was evaluated for four times．The maximum disease severity(1VlDS)on penultimate leaf was investigated in Anyaug under natural inoculation around May 18，2004 and 2005．With the method of composite interval mapping(CIM)，four quantitative trait loci(QTL)for APR to powdery mildew were detected fill chromosomes IAS，2BL，4BL’and 7DS，explaining 5．1％一26．6％ofphenotypic variance． The Q-IL ou chromosome IAS showed high gen甜c effect on powdery mildew resistance,accounting for 19．5％-26．6％of phenotyplc vaxiauce across two environments．The heritability of resistance to powdery mildew for maximum disease sevcrily(MDS)in two years and two locations ranged from 0．82 to 0．93．while the hexitabifity for AUDPc was between 0．84 and 0．91． The double haploid(DH)population with 104 lines derived from the cross Bainon964／Jingshuan916 was planted during 2005-06 cropping season in Beijing