流式细胞分析梅毒患者外周血T淋巴细胞的免疫表型x(精选).docxVIP

流式细胞分析梅毒患者外周血T淋巴细胞的免疫表型流式细胞分析梅毒患者外周血T淋巴细胞的免疫表型中华皮肤科杂志 1998年第3期第31卷 论著作者：钱黎华　王侠生　杨蜀嵋　徐金华　闫永宁　徐明单位：200040 上海医科大学华山医院皮肤科关键词：梅毒；淋巴细胞免疫表型；流式细胞计数【摘要】　目的　研究人类对梅毒螺旋体的细胞免疫反应特征，分析进入血液后的梅毒螺旋体对机体外周血中各淋巴细胞亚群及自然杀伤细胞(NK) 的影响情况。方法　通过流式细胞仪，运用标有异硫氰酸荧光素( FITC)或藻红素(PE)的CD单抗，分析了24例未经驱梅治疗过的早期梅毒患者(含泛发型皮疹、 掌跖型皮疹及无皮疹型各8例)外周血中各T淋巴细胞免疫表型的特点。结果 　24例梅毒患者的外周血中总T、TH、NK细胞及TH/Ts的比值均明显低于正常人对照组(P＜0.01)，而Ts的比例则显著高于正常人对照组(P＜0.01)；另外皮疹呈泛发型、掌跖型、无皮疹型三组患者在上述免疫表型的比例方面 相互比较，无统计学差异(P0.05)。结论　未经驱梅治疗过的早期梅毒患者存在着明显的细胞免疫抑制，其中皮疹呈泛发型、掌跖型、无皮疹型三者基本上反映了进入血液后的梅毒螺旋体在不同皮肤屏障功能下的表现形式，而与机体外周血中T淋巴细胞亚群的变化之间无明显的相关关系。Analysis of Peripheral Blood Lymphocyte Immunophenotypes in Syph ilitic Pat ients with Flow CytometryQian Lihua,Wang Xiasheng,Yang Shumei,et al. Departme nt of Dermatology,Huashan Hospital,Shanghai Medical University,Shanghai 200040 【Abstract】　Objective　To characterize the human cell-mediated immune response to Treponema pallidum(TP), and to determine the effect of TP in fection on the vari ous lymphocyte subpopulations and natural killer cells in the peripheral blood o f the patients. Methods Twenty-four untreated patients with ea rly syphilis were analyzed,including 3 groups of 8 patients with disseminated syphilides,palmoplantar syphilides and latent syphilis each. Monoclonal antibodies of CD labeled with either fluor escein isothiocyanate or phycoerythrin were used on a flow cytometry to detect t he chan ging of pe ripheral blood lymphocyte immunophenotypes. Results The results showed that the percentages of panT,TH,NK cells and the ratio of TH/TS were significant ly lower (P ＜0.01) and those of TS cells were significantly higher (P[ WTBZ ＜0.01) than those of normal controls. No statistical difference of t he immunophenotype distribution was found among groups of disseminated lesions, palmoplantar syphilides, and latent syphilis (P0.05).Conclusion It suggests that there is prominent suppression of c ellular immunity in the untreated patients suf fering from early syphilis. Meanwhile, the difference of 3 group