中药青蒿和青蒿素类药物抗肿瘤作用的实验研究.pdfVIP

广州中医药大学2015届硕士学位论丈 3．3小鼠EAC艾氏腹水瘤生存时间的影响结果显示，青蒿琥酯60mg／kg剂量腹腔注 射给药后的腹水瘤小鼠生存时问明显得到延长，并且不影响小鼠的生存质量，因此认 为通过腹腔注射方式给药，青蒿琥酯60mg／kg剂量可能是一个比较适宜的抗EAC腹水 瘤的实验剂量，值得进一步研究。 3．4中药青蒿与青蒿素类药物对H。。荷瘤小鼠脾淋巴细胞增殖及转化的影响的结 果表明，与模型对照组比较，青蒿素、青蒿琥酯、青蒿醇提物、青蒿水提物的各剂量 组均有显著差异 P O．01 ，提示能明显提高荷瘤小鼠的脾淋巴细胞转化功能 瘤小鼠的脾淋巴细胞转化功能。 关键词： 中药青蒿：青蒿素类药；抗肿瘤活性；抑瘤率；脾淋巴细胞 II of i——tumorEffectofArtemi si aannuaand The Ant study artemj si ni n ChineseMedicine i l Foundationoftraditional a Specty：Clinical Lime Author：Tan Tutor：Li Guoqiao Abstract 1 ObjectIve a Artemisiaannuabecomehotresearch，it antitumoractivitesof may The new anticancerthe ofanimal is tobe intoa drug．Byway hope developed plant effectofArtemiSiainvestigate observetheantitumor annua，to experiments，to andthe order betweenthese body’Simmune，in the relationship drugs possible ofits onthemechanism fortheFurtherdetermine to areference provide of basisforthenewuse old anti—tumor．Itsnext inresearchscientific step idea． to aresearch drugsopen 2Methods the oftotal 2．1UseUV—visible todeterminatecontent spectrophotometry Extractand flavonoidsandtotal inArtemasiaEthanol p01ysaccharides ArtemasiaWaterExtract． mice subcutaneousatthe Modeswere and injection 2．2 SlBo H22bearingby and dividedintoten underSPFWaSselectedrandomly mouseaxi11aKMmice wereCTX dosesofArtemisinin 150mg／kg， groups．they group，different ArtesunateOral 60mg／kg ， 300mg／kg ，Artesunateinjection 60mg／kg and andArtemasiaWater ofArtemasiaEthanolExtract differe．ntdoses control had Extract 1 group Model ，eachgroup 000mg／kg，4000mg／kg ，model was forlO 11edthesemice drugs days．Ki lOmice，24hlater，eachgroupgiven and were after inlastyen 24hLater．’[he thymusweighted gi drug tumor，spleen and tumor index dissectjontocalculateinhibitoryrate，spleen thymus complot index． mi aLthemouse ModeswasEAC ce subcutaneous 2．3 bearingby injection ax¨】aKMmiceunderSPFwasselectedand Videdintoten randomly 】i groups， ofArtemi CTX fferentdoses sinin 1 were they group．di Artesunate fferentdoses Artesunate