Lecture of all Nematode.pptVIP

Experimental Human Parasitology Lei Jiahui 雷家慧 Items clarified Morphological characters and techniques required Wearing lab coat Slides collected in appointed containers Clean work area and wash your hands Assignments after observation SECTION 1 Nematode Ⅰ Hookworm（钩虫）: Ancylostoma duodenale 十二指肠钩口线虫 Necator americanus 美洲板口线虫 Ascaris lumbricoides 似蚓蛔线虫 Trichuris trichiura 毛首鞭形线虫 ? Aims and Demands Morphological characters of the 4 kinds of eggs Morphological characters of all adults Operative procedures on direct smear（直接涂片法）, saturated brine method （饱和盐水浮聚法）and larval cultivation in a tube（小试管钩蚴培养法） Egg Fertilized egg of Ascaris lumbricoides Shape: ovoid Size: 60 μm x 45 μm Color: brownish Shell: thick , with brown protein coating Contents: oocyte, a semilunar space at each end Unfertilized egg of Ascaris lumbricoides more elongated in shape thinner shell and protein coating Contents: refractile granules Egg of Hookworm Shape: oval Size: mid-sized Color: transparent Shell: very thin Contents: 4-8 oocytes Egg of Trichuris trichiura Shape: spindly, with a plug at each pole Size: 25 μm—50μm Color: brown Shell: thick Contents: unsegmented oocyte Adult Adult of Ascaris lumbricoides The largest one among human intestinal nematodes Living: pink or reddish color Dead: whitish male: curved tail female: larger, conical and straight end A. Duodenale and N. americanus differ in: Shape Buccal capsule (口囊) and teeth Copulatory bursa (交合伞) Spicule (交合刺) Dorsal rays (背辐肋) Adult of Trichuris trichiura like a whip: anterior 3/5 elongated and thin, posterior 2/5 fleshy and bulbous Detection techniques Direct smear(直接涂片法) Prepare a clean slide and add 1-2 drops of normal saline in the middle of slide. Take a sample with a rice size from the stool specimen and put it in the area with normal saline, smearing well. It is necessary to make 3 slides for one specimen and