CCNG2基因过表达对甲状腺癌裸鼠移植瘤实验研究.docVIP

CCNG2基因过表达对甲状腺癌裸鼠移植瘤实验研究 　　【摘 要】 目的 利用人K1甲状腺癌移植型裸鼠模型，探讨细胞周期蛋白G2（cyclin G2）的体内抑制肿瘤效应。方法 将18只4周龄SPF级BALB/C雌性裸鼠编号，随机数字表法分为空白对照组、阴性转染对照组和CCNG2基因转染组，每组6只。常规扩大培养三种细胞，将生长状态良好的细胞消化后重悬于无血清培养基中，0.2ml/只（终浓度为5×107）于裸鼠右侧腋窝皮下按组别接种建立甲状腺癌动物模型，同时游标卡尺测量瘤体的长轴、短轴计算移植瘤体积，绘制生长曲线。30天后断颈处死所有组别的裸鼠，摘取瘤组织，免疫组织化学检测CCNG2蛋白的表达。结果 所有组别裸鼠全部成瘤，成瘤率100%（18/18）。CCNG2基因转染组细胞组裸鼠成瘤时间延迟、瘤组织体积及生长速度缓慢，平均体积明显小于空白对照组和阴性转染对照组（F=54.983，P0.001），体积抑瘤率分为67.07%、62.32%。免疫组织化学检测结果示：LV-CCNG2转染组染色阳性指数分别为9.79±0.658，与空白对照组和阴性转染对照组染色指数4.659±0.371和4.719±0.367相比，差异具有统计学意义（P0.05）。结论 慢病毒介导CCNG2过表达于人K1甲状腺癌细胞在裸鼠体内的生长受到了明显的抑制。 　　【关键词】 细胞周期蛋白G2（CCNG2）；甲状腺癌；K1细胞；慢病毒；裸鼠 　　The effects of human CCNG2 gene overexpress in nude mice of Thyroid carcinoma 　　LIANG Hui-Jie， LIU Ge-Ling 　　（1.Department of Endocrinology，the Workers hospital affiliated， North China university of 　　technology，Tangshan 063000，China；2. Department of Endocrinology，the Workers hospital，Tangshan 063000，China） 　　ABSTRACT：【Objective】To explore cyclin G2 in vivo antitumor effect in nude mice implanted with K1 thyroid cancer cells.【Methods】Choosing four-week-old SPF BALB/C female mice （n=18）. Then separated them into three groups at random： blank control group （K1）， LV-CCNG2 transfected group and negative control group . K1 thyroid cancer cells were culture， and 0.2ml （5x107/ml） of cell suspension were injected in to the subcutaneous tissue of back to establish BALB/C nude mice eth otopic transplantation tumor model. We measured the long and minor axis of the transplanted tumors using slide caliper， drew the growth curve of them. All groups of the nude mice were breaking the neck todeath on the 30th day. The expression CCNG2 was measured by IHC . To observe the inhibitory effect of CCNG2 over expression on tumor in vivo.【Result】The rate of tumorigenesis in each group was 100%. There was significant difference on tumor size in three groups （F=54.983，P 　　KEY WORDS： cyclin G2（CCNG2）；thyroid；K1 cell；lentivirus；Nude mice 　　甲状腺癌（Thyroid carcinoma，TC）是一种比较常见的恶性肿瘤，