精子DNA碎片率对女性复发性自然流产的影响.docVIP

精子DNA碎片率对女性复发性自然流产的影响 　　【摘要】目的：探讨精子DNA 碎片率与女性复发性自然流产的关系。方法：收集2013年6月至2013年12月在本中心就诊的发生过2次或2次以上自然流产的患者。将此配偶有不明原因复发性流产史的80例作为实验组；另外选择配偶无流产史的80例作为对照组。分别测定两组男方精子DAN碎片率。结果：实验组精子DNA碎片检出阳性32例，对照组精子DNA碎片检出阳性12例，有统计学差异（P0.05）。结论：复发性自然流产组精子DNA碎片率阳性检出率显著高于对照组，提示精子DNA碎片率较高可能跟早期复发性自然流产有关。 　　【关键词】复发性自然流产；精子DNA 碎片率；精子染色质扩散法 　　Influence of sperm DNA fragmentation on recurrent spontaneous abortionCAO Longqiao1， JIANG Yonghui2， WANG Xuesong1， SUN Xiuqin1. 1. Department of Reproductive Medicine， First People’s Hospital of Jining， Jining 272000， China； 2. Shandong University of Traditional Chinese Medicine， Jinan 250000， China 　　【Abstract】Objectives： To study the influence of the rate of sperm DNA fragmentation on recurrent spontaneous abortion. Methods： The samples of patients having had twice or more spontaneous abortion in this center from June 2013 to December 2013 were collected. The 80 patients had unexplained recurrent spontaneous abortions were selected as experimental group while the 80 ones without a history of abortion were chosen as the control group. The sperm DNA fragmentation rate of the two groups was evaluated. Results： There were 32 and 12 cases detected sperm DNA fragmentation positive in the experimental group and control group respectively， with statistically significant difference （P0.05）. Conclusions： The positive ratio of sperm DNA fragmentation in experiment group is significant higher than that of the control group. It implicates that increase in the sperm DNA fragmentation may lead to recurrent spontaneous abortion. 　　【Key words】Recurrent spontaneous abortion； Sperm DNA fragmentation； Sperm chromatin dispersion 　　【中图分类号】R169.4【文献标志码】A 　　复发性自然流产（recurrent spontaneous abortion， RSA）是指妊娠前20周连续发生2次及2次以上的胚胎停育，在女性中发生率为1%～5%， 其发病机制尚未完全阐明，可能与解剖异常、免疫失调、内分泌紊乱、染色体异常、感染、不良嗜好、血栓形成倾向甚至环境污染和基因多态性等有关。不明确的发病机制给临床治疗带来了困难[1-4]。目前的研究仍然主要集中在女性方面，即使做了详尽的病因筛查，仍有超过40%～60%的病例未找到原因。胚胎的染色体异常或基因异常是导致早期自然流产的主要原因，且流产次数与胚胎染色体或基因异常发生率之间无明显关联。外周血染色体核型分析仅能为8%的自然流产患者找到原因，这表明研究配子阶段的染