氧化槐定碱保护脑缺血后再灌注损伤的作用相当尼莫地平.docVIP

Protective effect of oxysophoridine on cerebral ischemia/reperfusion injury in mice Hongbo Wang1, Yuxiang Li2, 3, Ning Jiang3, Xiaoping Chen3, Yi Zhang3, Kuai Zhang1, Tengfei Wang1, Yinju Hao1, Lin Ma4, Chengjun Zhao5, Yanrong Wang6, Tao Sun4, Jianqiang Yu1, 7 1 Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China2 College of Nursing, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China3 Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China4 Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan 750004, Ningxia Hui Autonomous Region, China5 School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China6 Key Laboratory of Reproduction and Genetics of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China7 Collaborative Innovation Center of Ningxia Hui Autonomous Region for Medicines, Yinchuan 750004, Ningxia Hui Autonomous Region, China 摘要? 氧化槐定碱是从苦豆子中提取的生物碱，课题组的前期研究发现其对缺血性脑损伤有保护作用。本次实验采用线栓法阻塞小鼠大脑中动脉2 h后行再灌注制备局灶性脑缺血再灌注损伤模型，造模前7 d开始腹腔注射62.5，125，250 mg/kg氧化槐定碱或灌胃6 mg/kg尼莫地平，1次/d。再灌注后24 h取小鼠损伤脑组织进行检测。结果发现，125，250 mg/kg氧化槐定碱和尼莫地平均可降低模型小鼠的神经功能缺陷评分，减少模型小鼠脑梗死体积，降低模型小鼠损伤脑组织含水量，可见氧化槐定碱对脑缺血再灌注损伤具有保护作用。进一步探讨其相关机制发现，62.5，125，250mg/kg氧化槐定碱和尼莫地平均可增加模型小鼠损伤脑组织ATP含量，降低丙二醛和一氧化氮含量；提高损伤脑组织超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、乳酸脱氢酶活性，降低一氧化氮合成酶活性。同时，250 mg/kg氧化槐定碱和尼莫地平可明显抑制模型小鼠损伤脑皮质N-甲基-D-天冬氨酸受体1亚单位mRNA和蛋白表达。作者认为，氧化槐定碱对脑缺血再灌注损伤的保护作用与抗氧化应激、抑制N-甲基-D-天冬氨酸受体1亚单位表达有关。 关键词：?神经再生；?中医药；?脑损伤；?氧化槐定碱；?缺血再灌注损伤；?氧化应激；?N-甲基-D-天冬氨酸受体；?神经保护；?基金资助文章； Acceptance date:?2013-02-01 Cite this article: Hongbo Wang, Yuxiang Li, Ning Jiang, Xiaoping Chen, Yi Zhang, Kuai Zhang, Tengfei Wang, Yinju Hao, Lin Ma, Chengjun Zhao, Yanrong Wang, Tao Sun, Jianqiang Yu. Protective effect of oxysophoridine on cerebral ischemia/reperfusion injury in mice[J].?Neural Regeneration Research, 2013, 8 15 : 1349-1359.