甘薯中5种有机磷农药残留的快速测定方法.docVIP

甘薯中5种有机磷农药残留的快速测定方法 摘要 建立了甘薯中敌敌畏、氧乐果、乐果、对硫磷、马拉硫磷共5种有机磷农药的气相色谱测定方法。样品经乙腈提取，DB-1701P毛细管柱色谱分离，FPD检测器检测，基质匹配外标法定量。5种有机磷农药在浓度0.05～1.00 mg/L范围内均有良好的线性关系，相关系数不低于0.999 6，加标回收率为78.2%～108.9%，相对标准偏差在（n 7）3.6%～10.4%之间。应用此法，5种有机磷农药在12 min内全部出峰。该方法具有简单、快速和定性准确等优点，满足残留量检测的要求。 关键词 气相色谱法；甘薯；有机磷农药；残留量 中图分类号 S531；X839.2 文献标识码 A 文章编号 1007-5739（2016）04-0118-02 Determination of 5 Organophosphorus Pesticides Residue in Sweet Potato by Using Gas Chromatography WU Xing-xiang 1 YANG Juan 1 LI Xiao-peng 2 * CHEN Nan 2 MA Shi-zhu 2 FENG Min-ling 3 （1 Sanshui Agricultural Products Quality and Security Inspection Center of Foshan City in Guangdong Province，Foshan Guangdong 528100； 2 Sanxiang Town District Inspection and Testing Station of Agricultural Products in Zhongshan City； 3 Zhongshan Quality Supervision Inspection Institute of Agricultural Products） Abstract A gas chromatography method was developed for the determination of 5 organophosphorus pesticides residues，including dichlorvos，omethoate，dimethoate，parathion，malathion in sweet potato sample. The samples were extracted with acetonitrile，then separated in a DB-1701P capillary column and detected with flame photometric detector（FPD）.A matrix matched external standard was used for quantitative analysis. The calibration curves were in good linearity between the peak area and the concentration of 0.05～1.00 mg/L for 5 organophosphorus pesticides with correlation coefficients not less than 0.999 6.The spiked recoveries ranged from 78.2% to 108.9% with RSD′s（n 7）in the range of 3.6%～10.4%. Using this method，5 organophosphorus pesticides were all found in 12 minutes. The proposed method was simple，fast and accurate，and it met the requirements of residue detection. Key words gas chromatography；sweet potato；organophosphorus pesticide；residue 甘薯，亦称番薯、白薯、红苕、山芋等，是一种重要的块根类植物。甘薯含有丰富的纤维素、蛋白质和多种维生素等。特别是甘薯含有丰富的赖氨酸，而大米、面粉恰恰缺乏赖氨酸。我国常年种植面积达666.67万hm2，总产量约1.5亿t，甘薯现已成为仅次于水稻的第二大粮食作物。现代营养学研究和营养学调查结果表明，甘薯不仅营养丰富，而且具有增强免疫调节、抑制癌症等功效[1]，近年来受到人们的热烈追捧。同时，它还是食品工业、轻工业