镉胁迫对生菜种子萌发及幼苗生理特性的影响.docVIP

镉胁迫对生菜种子萌发及幼苗生理特性的影响 　　摘要：水培条件下，以生菜（Lactuca sativa L.）种子为供试材料，研究0、5、10、20、40 mg/L镉（Cd2+）处理对生菜种子萌发及幼苗生理特性的影响。结果表明，低浓度Cd2+（5 mg/L）胁迫对生菜种子萌发具有明显的刺激作用，其中种子的发芽势较没有Cd2+胁迫处理的增加63.64%;发芽指数和活力指数均达到最大值，分别为40.30和119.29;而随着营养液中Cd2+浓度的进一步升高，种子的萌发表现出明显的抑制作用，发芽势、发芽率显著降低，发芽指数和活力指数逐步减小，且处理浓度越高种子发芽的抑制作用越明显。与没有Cd2+胁迫的处理相比，低浓度Cd2+（5 mg/L）胁迫对生菜幼苗生长也具有促进作用，根长、芽长、鲜质量增幅分别为16.04%、11.61%和11.59%，且生菜幼苗叶片中叶绿素a含量、叶绿素b含量以及叶绿素总量的水平均显著升高;当Cd2+浓度10 mg/L时，生菜幼苗生长明显受到抑制，且叶绿素a含量、叶绿素b含量以及叶绿素总量的水平均显著降低。相比较而言，Cd2+胁迫对生菜幼苗根生长的抑制作用大于对地上部的抑制作用，Cd2+胁迫对生菜幼苗叶片叶绿素a的影响作用小于对叶绿素b的影响。此外生菜幼苗根组织中丙二醛含量随Cd2+浓度的增大而显著增加。 　　关键词：镉胁迫;生菜（Lactuca sativa L.）;种子萌发;叶绿素;丙二醛 　　中图分类号：S604;S636.2 文献标识码：A 文章编号：0439-8114（2014）20-4892-05 　　DOI：10.14088/j.cnki.issn0439-8114.2014.20.031 　　Effects of Cadmium Stress on Seed Germination and Physiological 　　Characteristics of Lettuce Seedling 　　XU Jie， HU Bo-hua， GE Tao， CHEN Qin 　　（College of Biological， Chemical Sciences and Engineering， Jiaxing University， Jiaxing 314001， Zhejiang， China） 　　Abstract： Effects of cadmium stress on seed germination and physiological characteristics of lettuce seedling were studied under five Cd2+ concentrations （0， 5， 10， 20， 40 mg/L） with solution culture. The results showed that low Cd2+ concentration stress（5 mg/L）significantly promoted the seed germination. The germination energy was increased by 63.64 % compared with the treatment with no Cd2+. Both the germination index and the vigor index reached the maximum of 40.30 and 119.29， respectively. The seed germination was significantly inhibited with the increase of Cd2+ concentration. The germination energy and the germination rate significantly decreased. The germination index and vigor index decreased gradually. The higher Cd2+ concentration， the more obvious inhibition of seed germination. The growth of lettuce seedling was promoted over 5 mg/L Cd2+ concentration compared with the treatment with no Cd2+. The increasing amplitude of root length， bud length and fresh weight was in the order o