裸花紫珠试管苗生产技术初探.docVIP

裸花紫珠试管苗生产技术初探 　　摘要：以带腋芽的裸花紫珠（Callicarpa nudiflora Hook. et Arn.）嫩茎段为外植体，研究其组培快繁技术。结果表明，用0.1%的升汞处理外植体15 min， 接种于MS+1.5 mg/L 6-BA+30 g/L蔗糖培养基上，7 d后萌发腋芽；丛生芽在MS+2.0 mg/L 6-BA+30 g/L蔗糖的培养基增殖效果最佳，增殖系数达8.50；丛生芽的继代培养周期是30 d，超出40 d时会有死株现象；培养基MS+0.5～0.8 mg/L NAA+0.5 g/L活性炭+30 g/L蔗糖适宜诱导生根，生根率达100%；生根苗移栽在V河沙∶V木屑∶V园土=1∶1∶1的混合基质中，塑料薄膜保湿20 d成活率达93%，且植株生长良好。 　　关键词：裸花紫珠（Callicarpa nudiflora Hook. et Arn.）；试管苗；茎段；组织培养 　　中图分类号：S567.1+9；Q943.1 文献标识码：A 文章编号：0439-8114（2014）13-3121-03 　　Production Technologies of Callicarpa nudiflora Hook. et Arn. Test-Tube Plantlets 　　HUANG Sai，PAN Mei，QI Hua-sha，WANG Jing-fei，L？? De-ren 　　（Institute of Horticulture and Flower， Hainan Academy of Agricultural Sciences， Haikou 571100， China） 　　Abstract： Using tender stem segments of Callicarpa nudiflora as explants， the techniques of tissue culture and rapid propagation in vitro were studied. Results showed that the optimum disinfector was 0.1% HgCl2 for 15 min， with MS+6-BA1.5 mg/L+ sucrose 30 g/L as the initial medium. After inoculation for 7 d， the axillaries buds began to germinate. Using MS+6-BA 2.0 mg/L + sucrose 30 g/L as the proliferation medium， the proliferation multiples reached 8.50. The best cycle of subculture training was 30 d. The plantlets would appear dead when more than 40 d. The rooting culture medium was MS+NAA 0.5～0.8 mg/L+ active carbon 0.5 g/L + sucrose 30 g/L，with the best rooting effect and the rate of rooting of 100%. When the rooted seedlings were transplanted into the mediums of sand∶soil∶sawdust（1∶1∶1） and covered with plastic film for keeping moisture for 20 d， the survival rate reached up to 93%. 　　Key words： Callicarpa nudiflora Hook. et Arn.； test-tube plantlet； stem segment； tissue culture 　　裸花紫珠（Callicarpa nudiflora Hook. et Arn.）为马鞭草科紫珠属植物，是海南黎族医生常用药材之一。分布在海南的定安、儋州、澄迈、白沙、昌江、东方、三亚、陵水、保亭、琼中等地，尤其产于五指山的为上品[1]。具有抗菌、止血、散瘀消肿之功效，主治各种炎症、外伤出血、跌打肿痛、风湿肿痛、肺结核咳血、胃肠出血等症[2，3]。随着南药开发利用的不断深入，裸花紫珠的市场需求量不断增大，但供应渠道仍以野生采伐为主，不仅不能满足市场的需求，而且还导致了严重的生态问题。由于裸花紫珠的人工常规育种能力低，难于进行大规模繁殖育种，