维生素B1对黄独试管苗生长发育和生理生化指标的影响.docVIP

维生素B1对黄独试管苗生长发育和生理生化指标的影响 摘要：试验研究了不同浓度维生素B1对黄独 中图分类号：Q949.95 文献标识码：A 文章编号：0439-8114（2014）02-0359-03 Effects of Vitamin B1 on the Growth and Development and Physiological and Biochemical Indexes of Dioscorea bulbifera L. Plantlets HONG Sen-rong （College of Life Sciences， Shangrao Normal University， Shangrao 334001，Jiangxi， China） Abstracts： The effects of Vitamin B1 on the growth and development and physiological and biochemical indexes of Dioscorea bulbifera L. plantlets were studied. The results showed that when vitamin B1 concentration was 0.25 mg/L， the number of bud， the bud length， the root number， the root length， the leaf number， the leaf length and the plant height could increase the number of Dioscorea bulbifera L. plantlets. When the concentration of vitamin B1 increased， the morphological indexes of Dioscorea bulbifera showed a decreasing trend. The results of physiological and biochemical indexes determination showed that when vitamin B1 concentration was 0.25 mg/L， the total chlorophyll content and the activity of POD and SOD increased， but the contents of malonaldehyde（MAD） and proline， the membrane permeability decreased. When the concentration of vitamin B1 increased， the total chlorophyll content and the activity of POD and SOD decreased， while the contents of MAD and proline， the membrane permeability increased. Under the conditions of containing vitamin B1， the content of proline of plantlets was significantly positively correlatied with the content of MDA and themembrane permeability， but there was significant negative correlation between the total content of chlorophyll and the activity of POD and SOD. The changes of content of proline can be used as a physiological index to determine the growth and development inhibition degree of D. bulbifera L. plantlets.. Key words： vitamin B1； Dioscorea bulbifera L.； plantlet； growth and development； physiological and biochemical indexes 黄独（Dioscorea bulbifera L.）为薯蓣科薯蓣属植物，是世界广布种，在中国主要分布于浙江、江西、福建等地[1]。 1.1