- 1、本文档共18页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
VibrioparahaemolyticusDisruptionofEpithelialCellTight.ppt
Vibrio parahaemolyticus Disruption of Epithelial Cell Tight JunctionsOccurs Independently of Toxin Production Tarah Lynch, Scott Livingstone, Enrico Buenaventura, Erika Lutter, Jason Fedwick, Andre G. Buret, David Graham, and Rebekah DeVinney INFECTION AND IMMUNITY, Mar. 2005, p. 1275–1283 Background Vibrio parahaemolyticus Toxin production: TDH(thermostable direct hemolysin) TRH(TDH-related hemolysin) Barrier function is maintained by tight junction Tight junction comprise of transmembrane proteins (ex: actin、occludin、ZO-1) Cytoskeletal components are anchored to tight junction Background Latex agglutination test Background Lactate dehydrogenase (LDH) Background TER ( transepithelial resistance ) paracellular permeability Background Southern confirmation Discussion 破壞上皮的 barrier function 產物 TDH TRH 不是主要的腳色,可能有其他未知物質 Discussion 高濃度 TDH( 5 HU/ml) 一樣會造成TER↓,並增加 LDH release,所以並不排除 TDH and/or TRH 的毒性,只是作用較晚 非 TDH and/or TRH 的毒素,影響上皮的 barrier function 作用較早 (1.5 ~ 3 hr postinfection) Discussion 不同的 toxin genotypes 腸炎弧菌,在上皮的 barrier function 有相同的反應,說明可能是由其他物質造成的 當測 LDH release 指出破壞上皮的 barrier function 跟 cytotoxicity 無關,LDH release 測得於 cell lysis,但不能決定 cell 開始 apoptosis Summary Summary * -/+ +/- -/- +/+ PCR Southern confirmation TLH TRH TDH TDH / TRH +/+ +/- +/- -/+ -/- Purified TDH Uninfected Uninfected TDH(-) / TRH(-) toxigenic nontoxigenic -/+ +/- +/+ +/- -/- V. parahaemolyticus disrupts TER and that this disruption occurs independently of toxin production. +/+ +/- -/+ -/- -/- -/- -/- TER ↓ ? paracellular permeability ↑ V. parahaemolyticus disrupts TER and that this disruption occurs independently of toxin production. -/- +/+ -/- -/- red/green red/green V. parahaemolyticus disrupts TER and that this disruption occurs independently of toxin production. +/+ +/- +/- -/+ -/- -/- -/- -/- White: 3 hr Black: 6 hr Disruption of epithelial barrier function does not correlate with cytotoxicity V. parahaemolyticus disrupts TER and that this disruption occurs ind
文档评论(0)