蛋白质分选与膜泡运输-任丽.ppt

第二节细胞内膜泡运输 要点回顾 膜泡运输概观 COPⅡ包被膜泡的装配与运输 COPⅠ包被膜泡的装配与运输 网格蛋白/接头蛋白包被膜泡的装配与运输 转运膜泡与靶膜的锚定和融合 课后思考题 何谓分泌型蛋白合成的信号肽学说，涉及的主要组分如何协同作用？ 试述分泌蛋白的合成、加工及转运途径。 试述细胞内膜泡运输的概况、类型及其各自主要功能。 怎样理解细胞结构组装的生物学意义？ 扩展阅读 Dolezal P, et al. Evolution of the molecular mechines for protein import into mitochondria. Science, 2006, 313: 314-318. Edeling MA, et al. Life of a clathrin coat: insights from clathrin and AP structures. Nat Rev Mol Cell Biol, 2006, 7: 32-44. Gevorkyan-Airapetov L, et al. Interaction of Tim23 with Tim50 is essential for protein translocation by the mitochondrial TIM23 complex. J Biol Chem, 2009, 284: 4865-4872. Grosshans BL, et al. Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci USA, 2006, 103: 11821-11827. Gurkan C et al. The COP II cage: unifying principles of vesicle coat assembly. Nat Rev Mol Cell Biol, 2006, 7: 727-738. Jahn R, Scheller RH. SNAREs–engines for membrane fusion. Nat Rev Mol Cell Biol, 2006, 7: 631-643. Martens S, McMahon HT. Mechanisms of membrane fusion: disparate players and common principles. Nat Rev Mol Cell Biol, 2008, 9: 543-556. Mokranjac D, Neupert W. Protein import into mitochondria. Biochem Soc Trans, 2005, 33: 1019-1023. Neupert W, Herrmann JM. Translocation of proteins into mitochondria. Annu Rev Biochem, 2007, 76: 723-749. Soll J, Schleiff E. Protein import into chloroplasts. Nat Rev Mol Cell Biol, 2004, 5: 198-208. Wicknelr W, Schekman R. Protein translocation across biological membranes. Science, 2005, 310:1452-1456. Mellman, I. Nelson, W.J. Coordinated protein sorting, targeting and distribution in polarized cells. Nat. Rev. Mol. Cell Biol, 2008, 9: 833-845. Zanetti, G., Pahuja, K.B., Studer, S., Shim, S. Schekman, R. COPII and the regulation of protein sorting in mammals. Nat. Cell Biol, 2012, 14: 20-28 DAngelo, G., et al. Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi. Nature, 2013, 501: 116-120. Lev, S. Non-vesicular lipid transport by lipid-transfer pro