大鼠胆汁中酸枣仁黄酮提取物的定性分析.docVIP

大鼠胆汁中酸枣仁黄酮提取物的定性分析 摘要：定性分析酸枣仁黄酮提取物在大鼠胆汁中代谢成分。大鼠口服酸枣仁黄酮提取物后行胆管插管术，于0-34h内收集胆汁，采用高效液相色谱―离子阱质谱法定性分析其代谢物成分。结果显示胆汁中两种原形成分和四种代谢陈分得到推定。HPLC-DAD-MSn可用于定性分析酸枣仁黄酮提取物在大鼠体内的代谢过程，其代谢成分主要以原形形式存在，并可能与其代谢产物协同内源性成分发挥药理活性。 关键词：酸枣仁黄酮提取物 胆汁 代谢物 HPLC-DAD-MSn Doi：10.3969/j.issn.1671-8801.2013.07.001 Qualitative analysis of flavonoid metabolites in rat bile after oral administration of Semen Ziziphi Spinosae extract by HPLC-DAD-MSn Bao Kangde Zhao Jihong Qi Lianwen Abstract： The aim of this study was to establish a method for qualitative analysis of the metabolites in rat bile after oral administration of Semen Ziziphi Spinosae flavonoid extract. A cystic duct encheiresis was carried out on a SD rat to collect the bile from zero to 34th hour after oral administration of Semen Ziziphi Spinosae flavonoid extract. High performance liquid chromatography-ion-trap mass spectrometry was applied for qualitative analysis of metabolite composition. The results showed that two original flavonoids （spinosin and 6-feruloylspinosin） and four metabolic components in the bile samples were detected. The method of HPLC-Diode-Array Detection （DAD） and Ion-Trap Mass Spectrometry （MSn） （HPLC-DAD-MSn ） could be used to qualitatively analyze the metabolism of Semen Ziziphi Spinosae flavonoid extract in rat. Original flavonoids are the main metabolite compositions， and the pharmacological activities may be the result of synergistic effects of original flavonoids， other metabolites and / or endogenous components. Keywords：Flavonoid extract of Semen Ziziphi Spinosae （FES） Bile Metabolite HPLC-DAD-MSn 【中图分类号】R4 【文献标识码】A 【文章编号】1671-8801（2013）07-0001-02 酸枣仁为鼠李科植物酸枣（Ziziphous jujuba Mill.var.spinosa（Bunge）Hu ex H.F.Chow）的干燥成熟种子，具镇静、催眠和增强免疫等功效，系常用镇静安眠类中药[1，2]。近年来，科研人员对酸枣仁化学成分和药理作用进行的深入研究表明，酸枣仁中主含黄酮类、三萜皂苷类和脂肪油类成分，其中黄酮类成分在镇静和增强免疫等活性方面尤为突出[3]。在已报道的八种酸枣仁黄酮化合物中，spinosin（Sp.）和6-feruloylspinosin占其总量的60%以上[2，3]，然而对其在体内转化过程和作用机理研究相对较少，目前有关酸枣仁各有效活性部位的代谢过程研究仅见酸枣仁黄酮提取物在大鼠血浆、尿、粪样中代谢产物的HPLC-DAD-ITMS的定性分析[4]，本文采用高效液相色谱―离子阱质谱法对酸枣仁黄酮提取